目的 探討黃連解毒湯有效組分通過激活Caspase-11靶點抗膿毒癥的作用機制。方法 實驗分為脂多糖（LPS）組、對照組及黃連解毒湯上清液醇洗（HLJDT-7）40、80、120 mg/mL組和黃連解毒湯沉淀水洗（HLJDT-5）40、80、120 mg/mL組。MTT實驗檢測各組對細(xì)胞活力的影響，Western blotting檢測Caspase-1和Caspase-11蛋白在黃連解毒湯組分各濃度中的相對表達(dá)量。結(jié)果 黃連解毒湯組分對LPS處理的RAW264.7細(xì)胞進(jìn)行干預(yù)后能夠明顯的降低LPS對細(xì)胞的抑制。當(dāng)Caspase-11和Caspase-1蛋白敲除后，HLJDT-7 120 mg/mL組和HLJDT-5 40、80、120 mg/mL組對Caspase-11和Caspase-1蛋白表達(dá)水平具有明顯的抑制作用（P<0.05、0.01）。結(jié)論 黃連解毒湯有效組分可降低細(xì)胞內(nèi)的LPS，進(jìn)而抑制Caspase-11，降低Caspase-11介導(dǎo)的Caspase-1依賴的細(xì)胞焦亡作用。

Objective To investigate the mechanism of effective components of Huanglian Jiedu Decoction against sepsis by activating Caspase-11 target. Methods The experiment was divided into LPS group, control group, HLJDT-7 40, 80, and 120 mg/mL groups, and water extract of Huanglian Jiedu Decoction 40, 80, and 120 mg/mL groups. The effect of each group on cell viability was detected by MTT assay. The relative expression levels of Caspase-1 and Caspase-11 proteins in various concentrations of Huanglian Jiedu Decoction were detected by Western blotting. Results The intervention of Huanglian Jiedu Decoction on LPS-treated RAW264.7 cells could significantly reduce the inhibition rate of LPS on cells. When Caspase-1 and Caspase-11 proteins were knocked out, expressions of Caspase-11 and Caspase-1 proteins in HLJDT-7 120 mg/mL group and HLJDT-5 40, 80, and 120 mg/mL groups were significantly inhibited (P<0.05, 0.01). Conclusion The effective component of Huanglian Jiedu Decoction can reduce LPS in cells and inhibit Caspase-11, and reduce the pyroptosis mediated by Caspase-11 and dependent on Caspase-1.

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國家自然科學(xué)基金青年科學(xué)基金資助項目（81303205）；遼寧省高等學(xué)校創(chuàng)新人才計劃項目（LR2017002）；沈陽市中青年科技創(chuàng)新人才支持計劃項目（RC170344）；沈陽市科技計劃項目（18-013-078）