目的 探索芫花素對(duì)于MC3T3-E1細(xì)胞向成骨細(xì)胞分化的影響。方法 采用芫花素1、5、10、15 μmol/L處理MC3T3-E1細(xì)胞14 d，采用MTS法檢測(cè)芫花素對(duì)MC3T3-E1細(xì)胞增殖的抑制作用；實(shí)時(shí)定量PCR和Western blotting法檢測(cè)測(cè)芫花素對(duì)MC3T3-E1細(xì)胞中ALP、HDAC1和Runx2 mRNA和蛋白表達(dá)的影響。結(jié)果 芫花素5、10、15 μmol/L可以顯著促進(jìn)MC3T3-E1細(xì)胞中ALP、Runx2 mRNA和蛋白表達(dá)水平的提高（P<0.05），HDAC1 mRNA和蛋白表達(dá)水平的下調(diào)（P<0.05），表明芫花素可以促進(jìn)MC3T3-E1向成骨細(xì)胞分化。結(jié)論 芫花素具有促進(jìn)MC3T3-E1細(xì)胞可以促進(jìn)成骨細(xì)胞分化，對(duì)成骨細(xì)胞的分化過程有一定的調(diào)節(jié)作用。

Objective To study the effect of genkwanin on the differentiation of MC3T3-E1 cell into osteoblast. Methods The MC3T3-E1 cell was treated with genkwanin 1, 5, 10, and 15 μmol/L for 14 d. Inhibition of genkwanin on proliferation of MC3T3-E1 cell was detected by MTS method. Effect of genkwanin on ALP、HDAC1, and Runx2 mRNA and protein expressions of MC3T3-E1 cell was detected by the real-time quantitative PCR and Western blotting. Results Genkwanin 5, 10, and 15 μmol/L could promote the expression of ALP and Runx2 mRNA and protein expressions in MC3T3-E1 cells (P<0.05), but inhibit the expression of HDAC1 mRNA and protein expressions in MC3T3-E1 cells (P<0.05). Conclusion Genkwanin has the action of promoting the differentiation of MC3T3-E1 cell into osteoblasts, and can regulate the differentiation process of osteoblasts.

R966

天津市衛(wèi)生計(jì)生委、天津市中醫(yī)藥管理局中醫(yī)、中西醫(yī)結(jié)合課題資助項(xiàng)目（2017063）