目的 探討蘆丁通過抗氧化抗凋亡對蛛網(wǎng)膜下腔出血（SAH）大鼠早期腦損傷的保護(hù)作用。方法 頸內(nèi)動脈穿刺法構(gòu)建SAH模型。SD雄性大鼠分為假手術(shù)組、模型組和蘆丁50 mg/kg組，24 h后對SAH評分、神經(jīng)功能評分、腦含水量和伊文思藍(lán)滲出率進(jìn)行考察。并利用ELISA法檢測氧化應(yīng)激指標(biāo)丙二醛（MDA）、超氧化物歧化酶（SOD）、過氧化氫酶（CAT）和谷胱甘肽過氧化物酶（GSH-Px）的活性；并采用免疫熒光檢測調(diào)亡相關(guān)蛋白p53、Bax、caspase-3和Bcl-2的表達(dá)情況。結(jié)果 與模型組比較，蘆丁50 mg/kg組SAH評分明顯降低（P<0.05），神經(jīng)功能評分明顯提高（P<0.01）。與模型組比較，蘆丁50 mg/kg組大鼠左腦、右腦和小腦的腦水含量和伊文思藍(lán)滲出率明顯降低（P<0.05），提示蘆丁能夠減輕SAH大鼠血腦屏障的損傷。與模型組比較，蘆丁50 mg/kg組大鼠MDA水平顯著降低（P<0.05），SOD、CAT和GSH-Px水平顯著升高（P<0.05、0.01），提示蘆丁具有改善SAH大鼠氧化應(yīng)激的作用。蘆丁50 mg/kg給藥后p53、Bax和caspase-3表達(dá)量減少，Bcl-2表達(dá)增加。結(jié)論 蘆丁能夠通過抗氧化抗凋亡減輕SAH大鼠的早期腦損傷。

Objective To investigate the protective effect of rutin on early brain injury in mice with subarachnoid hemorrhage (SAH) by anti-oxidation and anti-apoptosis. Methods SAH model was established by internal carotid artery puncture. SD male mice were divided into Sham group, model group, and rutin 50 mg/kg group. After 24 h, SAH score, neurological function score, cerebral edema, and Evans blue exudation were investigated. The activities of oxidative stress indexes MDA, SOD, CAT, and GSH-Px were detected by ELISA method. The expression of apoptosis related proteins p53, Bax, caspase-3, and Bcl-2 were detected by immunofluorescence. Results Compared with the model group, SAH score in rutin 50 mg/kg group was significantly decreased (P<0.05), but neurological function score was significantly increased (P<0.01). Compared with the model group, cerebral edema and Evans blue exudation of left brain, right brain, and cerebellum in rutin 50 mg/kg group were significantly decreased (P<0.05), indicating that rutin could decrease the damage to the blood-brain barrier of mice with SAH. Compared with the model group, MDA level in rutin 50 mg/kg group was significantly decreased (P<0.05), but SOD, CAT, and GSH-Px levels were significantly increased (P<0.05, 0.01), indicating that rutin could improve the effect of oxidative stress in mice with SAH. Rutin 50 mg/kg could decrease the expression of p53, Bax, and caspase-3, and increase the expression of Bcl-2. Conclusion Rutin can alleviate early brain injury in mice with SAH by anti-oxidation and anti-apoptosis.

R966;R285.5

國家自然科學(xué)基金資助項(xiàng)目（81671174）