目的 優(yōu)選參黛愈瘍灌腸液的水提工藝。方法 以苦參堿、氧化苦參堿的提取率和干膏率為評價指標，采用信息熵理論確定各指標權重系數(shù)并計算綜合評分。以煎煮時間、加水倍數(shù)、煎煮次數(shù)為考察因素，采用L₉（3⁴）正交試驗篩選參黛愈瘍灌腸液的最優(yōu)提取工藝并進行驗證。結果 最優(yōu)的提取工藝為飲片煎煮3次，每次加8倍量水、每次煎煮1 h，苦參堿、氧化苦參堿的平均提取率分別為0.117 1%、0.010 8%，平均干膏率為36.53%。結論 優(yōu)選的水提工藝重復性好、穩(wěn)定可行，可為參黛愈瘍灌腸液的后續(xù)開發(fā)和工業(yè)化生產提供科學依據(jù)。

Objective To optimize the water extraction process of Shendai Yuyang Enema. Methods The extraction rates of matrine and oxymatrine and dry extractum were used as evaluation indexes. Information entropy theory was used to determine the weight of each indicator to calculate comprehensive score. L₉(3⁴) orthogonal design was used to screen the optimal extraction technology of Shendai Yuyang Enema with decoction times, water volume and decoction times as factor. Validation test was also performed. Results The optimal extraction process was extracting third times, adding 8 folds of water, and 1 h each time. The results of validation test showed that the average extraction rate of matrine and oxymatrine were 0.117 1% and 0.010 8%, respectively. The average dry extractum yield was 36.53%. Conclusion The optimized water extraction process is reproducible, stable and feasible. It can provide a scientific basis for the industrial extraction and preparation of Shendai Yuyang enema.

R943

國家中醫(yī)藥管理局全國名老中醫(yī)藥專家傳承工作室建設項目；深圳市科技計劃項目（ZDSYS201606081515458）