目的 探討紫鉚素通過(guò)免疫抑制功能對(duì)莫諾苯宗誘導(dǎo)白癜風(fēng)小鼠毛發(fā)脫色的改善作用。方法 40%莫諾苯宗乳膏誘導(dǎo)小鼠脫色構(gòu)建白癜風(fēng)模型，48只C57BL/6小鼠按體質(zhì)量隨機(jī)分對(duì)照組、模型組、他克莫司組和紫鉚素組，每組12只。模型組涂抹40%莫諾苯宗乳膏50 mg，1次/d，他克莫司組在模型組的基礎(chǔ)上7 h后涂抹0.1%他克莫司乳膏30 mg，1次/d；紫鉚素組在模型組的基礎(chǔ)上7 h后0.1%紫鉚素乳膏10 mg，1次/d，連續(xù)用藥50 d。觀察并記錄小鼠的毛發(fā)脫色情況；分離小鼠脾臟淋巴細(xì)胞，CFSE法檢測(cè)T淋巴細(xì)胞和B淋巴細(xì)胞增殖情況；采用ELISA法檢測(cè)各組小鼠血清中IgM、IgA和IgG水平；3H-TdR標(biāo)記法檢測(cè)IL-2分泌活性；流式細(xì)胞儀檢測(cè)表面抗原CD19活性。結(jié)果 與模型組比較，紫鉚素能顯著改善白癜風(fēng)小鼠毛發(fā)脫色現(xiàn)象，顯著降低脫色發(fā)生率、顯著減小脫色面積，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）；與模型組比較，紫鉚素能顯著抑制淋巴細(xì)胞增殖活性，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）；與模型組比較，紫鉚素能顯著降低小鼠血清中IgM、IgG、IgA水平（P<0.05），上調(diào)IL-2分泌活性和增強(qiáng)表面抗原CD19活性（P<0.05）。結(jié)論 紫鉚素可以改善白癜風(fēng)小鼠毛發(fā)脫色的現(xiàn)象，其作用機(jī)制可能是通過(guò)抑制小鼠自身免疫反應(yīng)。

Objective To investigate the improvement of butin on decolorization in vitiligo mice induced by monobenzone by inhibiting immune function. Methods 40% Monorubicin cream was used to induce decolorization of mice to construct vitiligo model. 48 C57BL/6 mice were randomly divided into control group, model group, tacrolimus group, and butin group according to the weight. Rats in the model group were applied with 40% Monobenzone Cream 50 mg, once daily. Rats in the tacrolimus group were applied with 0.1% Tacrolimus Cream 30 mg on the basis of model group after 7 h, once daily. Rats in the butin group were applied with 0.1% Butin Cream 10 mg on the basis of model group after 7 h, once daily. They were treated for 50 d. The hair discoloration of mice were observed and recorded. The spleen lymphocytes were isolated, and the proliferation of T lymphocytes and B lymphocytes were detected by CFSE assay. The levels of IgM, IgA and IgG in serum were detected by ELISA method. The secretion activity of IL-2 was detected by 3H-TdR labeling method, and the activity of CD19 was detected by flow cytometry. Results Compared with the model group, butin can significantly improve hair decolorization in mice with vitiligo, reduce the incidence and reduce the area of decolorization, with significantly difference between two groups (P<0.05). Compared with the model group, butin can significantly inhibit lymphocyte proliferation, with significantly difference between two groups (P<0.05). Compared with the model group, butin can significantly decrease IgM, IgG, and IgA levels in serum (P<0.05), and increase IL-2 secretion activity and surface antigen CD19 activity in serum (P<0.05). Conclusion Butin can improve the hair discoloration in mice with vitiligo, and its mechanism may be through inhibiting the autoimmune activity of mice.

R966;R285.5

國(guó)家自然科學(xué)基金青年科學(xué)基金資助項(xiàng)目（81803138、81502727）