目的 采用網(wǎng)絡(luò)藥理學(xué)方法研究人參-黃芪藥對(duì)治療2型糖尿病的作用機(jī)制。方法 利用中藥系統(tǒng)藥理學(xué)技術(shù)平臺(tái)（TCMSP）獲取人參-黃芪藥對(duì)的主要成分和對(duì)應(yīng)靶點(diǎn)，通過UniProt和DrugBank數(shù)據(jù)庫查詢靶點(diǎn)對(duì)應(yīng)的基因，運(yùn)用Cytoscape3.6.1構(gòu)建活性成分-靶點(diǎn)相互作用網(wǎng)絡(luò)。然后通過TTD、DigSee、CTD多個(gè)數(shù)據(jù)庫查詢2型糖尿病的相關(guān)靶點(diǎn)，與人參-黃芪藥對(duì)作用靶點(diǎn)取交集后獲得人參-黃芪藥對(duì)-T2DM疾病交集靶點(diǎn)。運(yùn)用STRING在線數(shù)據(jù)庫構(gòu)建蛋白相互作用（PPI）網(wǎng)絡(luò)，最后通過DAVID進(jìn)行GO功能和KEGG通路富集分析。結(jié)果 篩選得到41個(gè)活性化合物，對(duì)應(yīng)靶點(diǎn)219個(gè)，關(guān)鍵靶點(diǎn)涉及PTGS2、PTGS1、ADRB2、NCOA2、SCN5A等。PPI網(wǎng)絡(luò)包含36個(gè)蛋白，包括BCL2、CASP3、CASP8、TGFB1、NOS2、PPARG等。GO功能分析獲得269個(gè)條目（P<0.05），KEGG通路富集得到71條信號(hào)通路（P<0.05），包括胰島素抵抗通路、PI3K/Akt信號(hào)通路、脂肪細(xì)胞因子信號(hào)通路等。結(jié)論 人參-黃芪藥對(duì)治療2型糖尿病是多成分、多靶點(diǎn)、多通路協(xié)同作用，主要參與炎癥反應(yīng)、細(xì)胞凋亡、氧化應(yīng)激等發(fā)揮作用。

Objective To explore the mechanism of Ginseng Radix et Rhizoma and Astragali Radix drug pair in treatment of type 2 diabetes mellitus using network pharmacology. Methods The active compounds and targets of Ginseng Radix et Rhizoma and Astragali Radix drug pair were obtained by the analysis of traditional Chinese medicine system pharmacology platform (TCMSP), and UniProt and DrugBank databases were used to extract the gene names of the targets. Cytoscape3.6.1 software was used to construct the compound-target network. The disease targets corresponding to T2DM were obtained by using TTD, DigSee, and CTD databases. The Ginseng Radix et Rhizoma and Astragali Radix drug pair-T2DM disease intersection targets were obtained after intersecting with the target of Ginseng Radix et Rhizoma and Astragali Radix drug pair. PPI network was constructed by STRING online database, and gene ontology (GO) analysis of potential genes and pathway enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) were carried out using DAVID. Results A total of 41 kinds of effective compounds and 219 targets were selected. Key targets included PTGS2, PTGS1, ADRB2, NCOA2, and SCN5A etc. The PPI core network contained 36 protein, and key protein involved in BCL2, CASP3, CASP8, TGFB1, NOS2, and PPARG, etc. The functional analysis of GO obtained 269 GO items (P<0.05), and There were 71 signal pathways (P<0.05) in the KEGG pathway enrichment screening, involving insulin resistance, PI3K-Akt signaling pathway and adipocytokine signaling pathway and so on. Conclusion Ginseng Radix et Rhizoma and Astragali Radix drug pair is a complex process of multi-component, multi-target and multi-pathway in the treatment of type 2 diabetes mellitus, which mainly participate in inflammatory reaction, apoptosis and oxidative stress and so on.

R285.5

國家自然科學(xué)基金青年科學(xué)基金資助項(xiàng)目（81900468）；中華醫(yī)學(xué)會(huì)臨床藥學(xué)分會(huì)-吳階平基金會(huì)青年基金項(xiàng)目（LCYX-Q025）