目的 觀察天麻素對帕金森病小鼠紋狀體多巴胺（DA）、α-突觸核蛋白（α-Syn）和酪氨酸羥化酶（TH）的影響及神經(jīng)保護作用。方法 50只C57BL/6雄性小鼠通過ip 1-甲基-4-苯基-1，2，3，6-四氫吡啶（MPTP）建立帕金森病模型，分為模型組，天麻素低、中、高劑量組和多巴絲肼組，每組10只，另設(shè)對照組10只。天麻素低、中、高劑量組im 0.2、0.4、0.6 g/（kg·d） 天麻素注射液，多巴絲肼組im 0.05 g/（kg·d）多巴絲肼溶液，模型組、對照組im等量無菌生理鹽水，連續(xù)注射14 d。末次給藥后1、7、14 d對各組小鼠進行爬桿、懸掛、曠場實驗等行為學(xué)測試，14 d時處死，采用分光光度法檢測各組小鼠紋狀體內(nèi)丙二醛（MDA）和還原型谷胱甘肽（GSH）水平，Western blotting法檢測各組小鼠紋狀體內(nèi)α-Syn和TH表達，高效液相色譜–電化學(xué)檢測法檢測各組小鼠DA水平。結(jié)果 相同時間下，與對照組相比，模型組小鼠爬桿時間、紋狀體內(nèi)MDA、α-Syn水平顯著升高（P<0.05），懸掛評分、曠場活動總距離、曠場活動平均速度、紋狀體內(nèi)GSH、DA、TH水平顯著減少（P<0.05）；與模型組相比，天麻素0.2、0.4、0.6 g/（kg·d）組和多巴絲肼組小鼠爬桿時間、紋狀體內(nèi)MDA、α-Syn水平顯著減少（P<0.05），懸掛分值、曠場活動總距離、曠場活動平均速度、紋狀體內(nèi)GSH、DA、TH水平顯著增多（P<0.05），天麻素0.6 g/（kg·d）組與多巴絲肼組各指標比較差異均無統(tǒng)計學(xué)意義。結(jié)論 天麻素可能通過提高機體抗氧化能力，保護腦內(nèi)DA能神經(jīng)元，從而改善帕金森病小鼠運動障礙癥狀。

Objective To observe the effects of gastrodin on dopamine (DA), α-synuclein (α-Syn), and tyrosine hydroxylase (TH) in striatum of Parkinson's disease mice and its neuroprotection. Methods 50 C57BL/6 Male mice were ip with 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (MPTP) to establish the Parkinson's disease model, and they were divided into model group, gastrodin low, middle, high dose group, and madopar group, with 10 mice in each group, and another 10 mice in the control group. The low, middle, and high dose group were im 0.2, 0.4, and 0.6 g/(kg·d) Gastrodin Injection, respectively, and the madopar group was im 0.05 g/(kg·d) madopar solution, and the model group and the control group were im the same amount of sterile saline, continuous injected for 14 days. On the 1st, 7th, and 14th days after the last administration, the mice in each group were tested by climbing pole, hanging and open field experiments, and were killed at the 14th day, malondialdehyde (MDA) and reduced glutathione (GSH) levels in striatum were measured by spectrophotometry. Western blotting was used to detect the expressions of α-Syn and TH in striatum, and the content of DA in striatum of mice in each group was detected by high performance liquid chromatography electrochemical detection (HPLC- ECD). Results At the same time, compared with the control group, the climbing time, MDA, and α-Syn levels in striatum of the model group were significantly increased (P<0.05), but suspension score, total distance of open field activity, average speed of open field activity, levels of GSH, DA, and TH in striatum decreased significantly (P<0.05). Compared with the model group, the climbing time, MDA and α-Syn levels in striatum of the gastrodin 0.2, 0.4, and 0.6 g/(kg·d) groups and the madopar group were significantly decreased (P<0.05), but suspension score, total distance of open field activity, average speed of open field activity, levels of GSH, DA, TH in striatum increased significantly (P<0.05). There was no significant difference between the gastrodin 0.6 g/(kg·d) group and madopar group. Conclusion Gastrodin may protect the DA neurons in the brain by improving the antioxidant capacity of the body, so as to improve the motor disorders of Parkinson's disease.

R966

國家自然科學(xué)基金青年基金資助項目（81600940）