目的 利用超高壓色譜-四極桿-飛行時間串聯(lián)質(zhì)譜技術(shù)（UPLC-Q-TOF/MS）對大鼠ig北青龍衣乙醇提取物后在腦組織中成分分布進(jìn)行分析。方法 將北青龍衣醇提物按20 mL/kg ig大鼠后，采集腦組織，采用UPLC-Q-TOF/MS法分析；正負(fù)離子模式使用ESI源對數(shù)據(jù)進(jìn)行采集，并通過數(shù)據(jù)分析軟件進(jìn)行處理，對入腦成分分子式的鑒別采用精確質(zhì)量數(shù)、同位素峰度比比較進(jìn)行，進(jìn)一步將其二級質(zhì)譜圖與對照品或數(shù)據(jù)庫的二級譜圖進(jìn)行比對、結(jié)合已知的文獻(xiàn)報(bào)道和對化合物的裂解規(guī)律分析，確定入腦成分的結(jié)構(gòu)式。結(jié)果 從大鼠腦組織中鑒定了11種來源于北青龍衣的成分，包含7個原型成分、4個代謝產(chǎn)物，其中7個萘醌，2個二芳基庚烷，2個酚酸。結(jié)論 北青龍衣乙醇提取物在大鼠腦組織中分布的成分以萘醌類為主，為北青龍衣腦毒性的深入研究提供物質(zhì)基礎(chǔ)，也為其體內(nèi)成分、組織分布研究提供方法學(xué)借鑒。

Objective To research the components distribution in cerebrum tissue of ethanol extract of Jugland mandshurica Maxim based on UPLC-Q-TOF/MS technology. Methods After ig 20 mL/kg of alcohol extract from extract of J. mandshurica Maxim to rats, and the tissue samples of cerebrum collected from each rat. Using the UPLC-Q-TOF/MS to detect the substance, and the obtained data was processed by data analysis software. According to the accurate mass number and isotope abundance ratio of ion fragments, the secondary mass spectrometry comparison of the database and the analysis of cracking law, the chemical molecular formula of common ions was further confirmed. Results Eleven components were obtained, including 7 prototypic components and 4 metabolites, including 7 naphthoquinones, 2 diaryl heptanes, and 2 phenolic acids. Conclusion Naphthoquinones are the main components of extract of J. mandshurica Maxim in the brain of rats, which laid a foundation for the further study on the toxicity of the brain and the study on the distribution of components and tissues in vivo.

R969.1

國家自然科學(xué)基金資助項(xiàng)目（81503348）；黑龍江省自然科學(xué)基金資助項(xiàng)目（H2017044）