目的 建立高效液相色譜法同時(shí)測定活力蘇口服液中迷迭香酸、紫草酸、丹酚酸B、淫羊藿苷、寶藿苷I、二苯乙烯苷和虎杖苷。方法 采用Agilent Eclipse XDB-C₁₈色譜柱（250 mm×4.6 mm，5μm），流動相為乙腈-0.1%磷酸溶液，梯度洗脫，檢測波長280 nm，體積流量1.0 mL/min，柱溫30℃，進(jìn)樣量10 μL。結(jié)果 迷迭香酸、紫草酸、丹酚酸B、淫羊藿苷、寶藿苷I、二苯乙烯苷和虎杖苷分別在0.59~14.75、0.81~20.25、5.07~126.75、1.86~46.50、1.18~29.50、8.79~219.75、2.07~51.75μg/mL線性關(guān)系良好（r ≥ 0.999 1）；各成分平均加樣回收率分別為96.95%、97.80%、99.43%、98.53%、99.19%、100.01%、98.77%，RSD值分別為1.13%、1.28%、0.91%、0.88%、0.97%、0.62%、1.33%。結(jié)論 所建立的方法操作便捷，結(jié)果準(zhǔn)確，為全面評價(jià)活力蘇口服液的內(nèi)在產(chǎn)品質(zhì)量提供了科學(xué)依據(jù)。

Objective To establish an HPLC method for simultaneous determination of rosmarinic acid, lithospermic acid, salvianolic acid B, icariin, baohuoside Ⅰ, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin in Huolisu Oral Liquid. Methods Agilent Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm) was used for the separation. The mobile phase was consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min. The column temperature was 30℃, and the detection wavelength were set at 280 nm. Results Rosmarinic acid, lithospermic acid, salvianolic acid B, icariin, baohuoside Ⅰ, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin had good linear relationships in the ranges of 0.59-14.75, 0.81-20.25, 5.07-126.75, 1.86-46.50, 1.18-29.50, 8.79-219.75, and 2.07-51.75 μg/mL (r ≥ 0.999 1). The average recoveries of components were 96.95%, 97.80%, 99.43%, 98.53%, 99.19%, 100.01%, and 98.77% with the RSD values of 1.13%, 1.28%, 0.91%, 0.88%, 0.97%, 0.62%, and 1.33%, respectively. Conclusion The established method is convenient and accurate, which provides a scientific basis for the comprehensive evaluation of product quality of Huolisu Oral Liquid.

R286.02