18色譜柱（250 mm×4.6 mm，5μm）；以乙腈–0.1%甲酸水溶液為流動(dòng)相，梯度洗脫；檢測波長分別為330 nm（0～17 min檢測毛蕊花糖苷和焦地黃苯乙醇苷B1）、280 nm（17～50 min檢測迷迭香酸、紫草酸、丹酚酸B、2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷）；體積流量1.0 mL/min；柱溫30℃；進(jìn)樣量10μL。結(jié)果 毛蕊花糖苷、焦地黃苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷分別在1.23～30.75、0.59～14.75、0.71～17.75、1.47～36.75、9.78～244.50、6.57～164.25、1.19～29.75 μg/mL線性關(guān)系良好（r≥0.999 1）；平均回收率分別為99.14%、97.62%、96.84%、98.96%、100.11%、99.46%、98.92%，RSD值分別為1.15%、1.27%、1.02%、0.96%、0.65%、1.41%、0.87%。結(jié)論 該方法操作簡便、重復(fù)性好，為軟脈靈口服液中多指標(biāo)質(zhì)量評價(jià)和臨床應(yīng)用提供參考依據(jù)。;Objective To establish an HPLC method for determination of verbascoside, jionoside B1, rosmarinic acid, lithospermic acid, salvianolic acid B, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin in Ruanmailing Oral Liquid. Methods Venusil XBP C18 column (250 mm×4.6 mm, 5 μm) was used with acetonitrile - 0.1% formic acid as mobile phase with gradient elution. The detection wavelength were set at 330 nm (for verbascoside and jionoside B1) and 280 nm (for rosmarinic acid, lithospermic acid, salvianolic acid B,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin). The column temperature was 30 ℃, flow rate was set at 1.0 mL/min, and volume was 10 µL. Results Verbascoside, jionoside B1, rosmarinic acid, lithospermic acid, salvianolic acid B, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin showed good linear relationships within the ranges of 1.23 — 30.75, 0.59 — 14.75, 0.71 — 17.75, 1.47 — 36.75, 9.78 — 244.50, 6.57 — 164.25, and 1.19 — 29.75 μg/mL (r≥0.999 1). The average recoveries of those components were 99.14%, 97.62%, 96.84%, 98.96%, 100.11%, 99.46%, and 98.92% with the RSD values of 1.15%, 1.27%, 1.02%, 0.96%, 0.65%, 1.41%, and 0.87%, respectively. Conclusion The method is simple and repeatable, which provides reference for the quality evaluation and clinical application of Ruanmailing Oral Liquid."/>

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首頁 > 過刊瀏覽>2020年第35卷第12期 >2020,35(12):2321-2325. DOI:10.7501/j.issn.1674-5515.2020.12.006
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HPLC法測定軟脈靈口服液中毛蕊花糖苷、焦地黃苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2,3,5,4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷

Determination of verbascoside, jionoside B1, rosmarinic acid, lithospermic acid, salvianolic acid B, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin in Ruanmailing Oral Liquid by HPLC

發(fā)布日期：2020-12-22
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    [關(guān)鍵詞]
    [摘要]
    目的 建立HPLC法測定軟脈靈口服液中毛蕊花糖苷、焦地黃苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷。方法 采用Venusil XBP C18色譜柱（250 mm×4.6 mm，5μm）；以乙腈–0.1%甲酸水溶液為流動(dòng)相，梯度洗脫；檢測波長分別為330 nm（0～17 min檢測毛蕊花糖苷和焦地黃苯乙醇苷B1）、280 nm（17～50 min檢測迷迭香酸、紫草酸、丹酚酸B、2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷）；體積流量1.0 mL/min；柱溫30℃；進(jìn)樣量10μL。結(jié)果 毛蕊花糖苷、焦地黃苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2，3，5，4’-四羥基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷分別在1.23～30.75、0.59～14.75、0.71～17.75、1.47～36.75、9.78～244.50、6.57～164.25、1.19～29.75 μg/mL線性關(guān)系良好（r≥0.999 1）；平均回收率分別為99.14%、97.62%、96.84%、98.96%、100.11%、99.46%、98.92%，RSD值分別為1.15%、1.27%、1.02%、0.96%、0.65%、1.41%、0.87%。結(jié)論 該方法操作簡便、重復(fù)性好，為軟脈靈口服液中多指標(biāo)質(zhì)量評價(jià)和臨床應(yīng)用提供參考依據(jù)。
    [Key word]
    [Abstract]
    Objective To establish an HPLC method for determination of verbascoside, jionoside B1, rosmarinic acid, lithospermic acid, salvianolic acid B, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin in Ruanmailing Oral Liquid. Methods Venusil XBP C18 column (250 mm×4.6 mm, 5 μm) was used with acetonitrile - 0.1% formic acid as mobile phase with gradient elution. The detection wavelength were set at 330 nm (for verbascoside and jionoside B1) and 280 nm (for rosmarinic acid, lithospermic acid, salvianolic acid B,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin). The column temperature was 30 ℃, flow rate was set at 1.0 mL/min, and volume was 10 µL. Results Verbascoside, jionoside B1, rosmarinic acid, lithospermic acid, salvianolic acid B, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin showed good linear relationships within the ranges of 1.23 — 30.75, 0.59 — 14.75, 0.71 — 17.75, 1.47 — 36.75, 9.78 — 244.50, 6.57 — 164.25, and 1.19 — 29.75 μg/mL (r≥0.999 1). The average recoveries of those components were 99.14%, 97.62%, 96.84%, 98.96%, 100.11%, 99.46%, and 98.92% with the RSD values of 1.15%, 1.27%, 1.02%, 0.96%, 0.65%, 1.41%, and 0.87%, respectively. Conclusion The method is simple and repeatable, which provides reference for the quality evaluation and clinical application of Ruanmailing Oral Liquid.
    [中圖分類號]
    R286.02
    [基金項(xiàng)目]
    安徽省教育廳高校自然科學(xué)研究項(xiàng)目（KJ2017A770）