目的 采用網(wǎng)絡藥理學方法，科學闡釋腎康注射液治療慢性腎功能衰竭（CRF）的作用機制。方法 通過TCMSP數(shù)據(jù)庫獲取腎康注射液的活性成分、潛在藥物靶點，通過OMIM、TTD、GeneCards數(shù)據(jù)庫獲得CRF的相關靶點，并結(jié)合相關軟件進行網(wǎng)絡藥理學分析，包括蛋白質(zhì)-蛋白質(zhì)相互作用（PPI）、基因本體（GO）分析、京都基因和基因組百科全書（KEGG）分析。結(jié)果 從TCMSP數(shù)據(jù)庫共篩選得到腎康注射液活性成分96個，包括槲皮素、山柰酚、木犀草素等核心成分，涉及相關基因靶點244個。PPI網(wǎng)絡涉及93個藥物靶點，度值排名前10的核心靶點分別是AKT1、VEGFA、IL6、TNF、TP53、MAPK1、CASP3、EGF、MMP9、EGFR。GO功能分析共獲得710個條目（P<0.05），KEGG通路富集得到112條信號通路（P<0.05），包括PI3K-Akt、TNF、HIF-1等信號通路。結(jié)論 腎康注射液治療CRF是多成分、多靶點、多通路的綜合效應。

Objective Using network pharmacology, scientifically explain the mechanism of Shenkang Injection in the treatment of chronic renal failure (CRF). Methods Active ingredients and potential drug targets of Shenkang Injection were obtained through TCMSP database, CRF related targets were searched through OMIM, TTD, GeneCards databases, and perform network pharmacological was analyzed by related software, including protein-protein interaction (PPI), Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results A total of 96 active ingredients of Shenkang Injection were screened from the TCMSP database, including core ingredients such as quercetin, kaempferol, luteolin, and 244 related gene targets. The PPI network involves 93 potential drug targets, and the top 10 core targets were AKT1, VEGFA, IL6, TNF, TP53, MAPK1, CASP3, EGF, MMP9, and EGFR. GO function analysis obtained a total of 710 entries (P<0.05), 112 signal pathways of KEGG pathway enrichment were obtained (P<0.05), including PI3K-Akt, TNF, HIF-1 and other signal pathways. Conclusion The treatment of CRF with Shenkang Injection is a comprehensive effect of multiple components, multiple targets and multiple pathways.

R285.5

國家自然科學基金青年科學基金資助項目（81900468）；河南省醫(yī)學科技攻關計劃聯(lián)合共建項目（LHGJ20190273）