18色譜柱(250 mm×4.6 mm,5 μm);流動相:80%甲醇-10 mmol/L甲酸銨,梯度洗脫;體積流量:1.0 mL/min;柱溫:40 ℃;CAD霧化器溫度:35 ℃,采集頻率:10 Hz,過濾常數(shù):5.0;進(jìn)樣體積:20 μL;稀釋劑:70%甲醇。結(jié)果 綠原酸、阿魏酸、阿魏酸松柏酯、洋川芎內(nèi)酯A、Z-藁本內(nèi)酯、歐前胡素、異歐前胡素的質(zhì)量濃度分別在0.51~10.20 μg/mL(r=0.999 4),0.53~10.60μg/mL(r=0.999 3),2.52~50.40μg/mL(r=0.999 5),5.05~101.00μg/mL(r=0.999 4),5.01~100.20μg/mL(r=0.999 8),1.02~20.40μg/mL(r=0.9995)、0.62~12.40 μg/mL(r=0.9993)線性關(guān)系良好;平均回收率分別為99.46%、98.65%、98.48%、98.74%、98.60%、98.69%、98.58%,RSD值分別為1.62%、0.87%、1.03%、1.20%、1.25%、0.69%、0.66%。結(jié)論 方法準(zhǔn)確、重復(fù)性好,為川芎茶調(diào)散的質(zhì)量研究提供了更加全面的方法。;Objective To develop an HPLC-CAD method for simultaneous determination of chlorogenic acid, ferulic acid, coniferly ferulate, senkyunolide A, Z-ligustilide, imperatorin, and isoimperatorin in Chuanxiong Chatiao Powder. Methods Chromatographic separation was performed on a Capcell Pak MGⅡC18 (250 mm×4.6 mm, 5 μm) column with 80% methanol - 10 mmol/L ammonium formate as the mobile phase for gradient elution. The flow rate was 1.0 mL/min, and the column temperature was maintained at 40 ℃. The nebulization temperature of detector was 35 ℃, the acquisition frequency was 10 Hz, and the filtration constant was 5.0. Injection volume was 20 μL, and diluent was used as 70% methanol. Results Chlorogenic acid, ferulic acid, coniferly ferulate, senkyunolide A, Z-ligustilide, imperatorin, and isoimperatorin had good linear in the ranges of 0.51 — 10.20 μg/mL (r = 0.999 4), 0.53 — 10.60 μg/mL (r = 0.999 3), 2.52 — 50.40 μg/mL (r = 0.999 5), 5.05 — 101.00 μg/mL (r = 0.999 4), 5.01 — 100.20 μg/mL (r= 0.999 8), 1.02 — 20.40 μg/mL (r = 0.999 5), and 0.62 — 12.40 μg/mL (r =0.9993), respectively. The average recoveries were 99.46%, 98.65%, 98.48%, 98.74%, 98.60%, 98.69%, and 98.58% with RSD values of 1.62%, 0.87%, 1.03%, 1.20%, 1.25%, 0.69%, and 0.66%, respectively. Conclusion The method is accurate and reproducible to provide a more comprehensive method for the quality control of Chuanxiong Chatiao Powder."/>

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首頁 > 過刊瀏覽>2021年第36卷第6期 >2021,36(6):1138-1143. DOI:10.7501/j.issn.1674-5515.2021.06.007
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HPLC-CAD法測定川芎茶調(diào)散中綠原酸、阿魏酸、阿魏酸松柏酯、洋川芎內(nèi)酯A、Z-藁本內(nèi)酯、歐前胡素和異歐前胡素

Determination of chlorogenic acid, ferulic acid, coniferly ferulate, senkyunolide A, Z-ligustilide, imperatorin, and isoimperatorin in Chuanxiong Chatiao Powder by HPLC-CAD

發(fā)布日期:2021-06-26