目的 建立HPLC法同時測定炎可寧膠囊中鹽酸小檗堿、黃芩苷、大黃酸、大黃素、大黃酚、大黃素甲醚。方法 以Supelco Discovery C₁₈（250 mm×4.6 mm，5 μm）為色譜柱，甲醇–0.1%磷酸水溶液為流動相，梯度洗脫，檢測波長為280 nm，柱溫30℃，體積流量為0.8 mL/min，進樣量10 μL。結果 6種成分在各自線性范圍內關系良好（r²≥0.999），平均加樣回收率99.0%～105.1%，RSD 1.41%～2.36%。結論 該方法簡便準確，重復性好，可用于炎可寧膠囊的質量控制。

Objective To establish an HPLC method for the simultaneous determination of berberine hydrochloride, baicalin, rhein, emodin, chrysophanol, and emodin methyl ether in Yankening Capsules. Methods The Supelco Discovery C₁₈ (250 mm×4.6 mm, 5 μm) was used as the chromatographic column with mobile phase of methanol-0.1% phosphoric acid aqueous solution in gradient elution. The detection wavelength was set at 280 nm. The flow rate was 0.8 mL/min and the column temperature was 30℃. Results The six components had a good relationship within their respective linear ranges (r² ≥ 0.999). The average recovery rate of sample addition was 99.0%-105.1%, and the RSD was 1.41%-2.36%. Conclusion The method is simple, accurate, reproducible, and can be used for the quality control of Yankening Capsules.

R284.1