目的 對甘草瀉心湯中化學(xué)成分進(jìn)行鑒定。方法 對甘草瀉心湯、單味藥湯劑和混合對照品進(jìn)行HPLC分析。采用Agela venusil MP C₁₈（2）色譜柱（250 mm×4.6 mm，5 μm）；流動相：0.2%甲酸水溶液–乙腈，梯度洗脫，檢測波長265、327 nm，體積流量1.0 mL/min，柱溫25℃，進(jìn)樣量10 μL。液路采用三通分流0.2 mL/min進(jìn)入ESI源，在正、負(fù)離子模式下進(jìn)行質(zhì)譜檢測。采用甲酸鈉溶液作為內(nèi)參校準(zhǔn)液每針校正。根據(jù)對照品、文獻(xiàn)報(bào)道和數(shù)據(jù)庫數(shù)據(jù)推測鑒定相關(guān)色譜峰的化合物和單味藥來源。結(jié)果 提取出85個(gè)基峰離子，根據(jù)元素分析推測出其化學(xué)式，其中80個(gè)推測出了可能的化合物結(jié)構(gòu)信息，采用對照品確證了6個(gè)成分，其中歸屬于甘草、黃芩、大棗、黃連、干姜、半夏分別為40、31、13、11、5、1個(gè)成分。結(jié)論 HPLC-QTOF/MS作為一種高選擇性、高分離度、高靈敏度的分析手段能有效地鑒定甘草瀉心湯中化學(xué)成分。

Objective To identify the chemical constituents in Gancao Xiexin Decoction. Methods GancaoXiexin Decoction, single herb decoction and mixed reference substance were analyzed on HPLC C₁₈ column (250 mm×4.6 mm, 5 μm) with the mobile phase 0.2% formic acid- acetonitrile at a flow rate of 1.0 mL/min. Detection wavelengths were set at 265 and 327 nm, volume flow rate was 1.0 mL/min, column temperature was 25 ℃, and sample injection volume was 10 μL. The fluid was infused into the ESI source at a flow rate of 0.2 mL/min by a tees，and was scanned in positive and negative ion mode by MS2. Sodium formate solution was used as internal standard. The compound attribution of related chromatographic peaks and the source of single herb were deduced and identified by comparison with the standard, literature, and database. Results Base peak chromatogram ions (85) were extracted and chemical formulas were deduced by elemental analysis, of which 80 constituents were deduced, and 6 constituents were confirmed using reference substances. Glycyrrhizae Radix et Rhizoma, Scutellariae Radix, Jujubae Fructus, Coptidis Rhizoma, Zingiberis Rhizome, and Pinelliae Rhizoma contributed 40, 31, 13, 11, 5, and 1 constituents, respectively. Conclusion HPLC-QTOF/MS can be applied in identification of constituents in Gancao Xiexin Decoction as a high selectivity, high resolution, and high sensitivity method.

R286.02

全國中藥特色技術(shù)傳承人才培訓(xùn)項(xiàng)目（國中醫(yī)藥人教函[2018]204號）；第六批全國老中醫(yī)藥專家學(xué)術(shù)經(jīng)驗(yàn)繼承工作；天津市衛(wèi)健委中醫(yī)、中西醫(yī)結(jié)合科研基金項(xiàng)目（2019039）；天津市衛(wèi)健委中醫(yī)、中西醫(yī)結(jié)合科研基金項(xiàng)目（2019050）；天津中醫(yī)藥大學(xué)第二附屬醫(yī)院橫向科研項(xiàng)目（20191201）