目的 采用高效液相色譜（HPLC）法同時測定抗婦炎膠囊中木蘭花堿、黃柏堿、藥根堿、巴馬汀、小檗堿、槐果堿、苦參堿、氧化槐果堿、槐定堿和氧化苦參堿10種活性成分。方法 采用InerSustain AQ-C₁₈色譜柱（250 mm×4.6 mm，5 μm），流動相A：乙腈–無水乙醇（80∶20），流動相B：0.1%磷酸溶液，梯度洗脫，檢測波長220 nm，體積流量1.0 mL/min，柱溫30℃，進(jìn)樣量10 μL。結(jié)果 木蘭花堿、黃柏堿、藥根堿、巴馬汀、小檗堿、槐果堿、苦參堿、氧化槐果堿、槐定堿和氧化苦參堿分別在2.69～134.50、1.95～97.50、0.63～31.50、0.86～43.00、11.95～597.50、0.59～29.50、6.08～304.00、4.85～242.50、1.66～83.00、19.79～989.50 μg/mL線性關(guān)系良好（r≥0.999 3）；平均回收率分別為99.11%、98.23%、96.95%、97.78%、100.02%、97.21%、99.66%、99.52%、98.81%、100.08%，RSD值分別為1.04%、1.23%、1.37%、1.65%、0.70%、1.28%、0.65%、0.81%、1.11%、0.63%。結(jié)論 建立的HPLC法可用于抗婦炎膠囊中10種活性成分的測定，作為抗婦炎膠囊質(zhì)量控制方法。

Objective To develop an HPLC method for the simultaneous determination of 10 active components in Kangfuyan Capsules, including magnoliatine, phellodendrine, jatrorrhizine, palmatine, berberine, sophocarpine, matrine, oxysophocarpine, sophoridine, and oxymatrine. Methods The separation was performed on an InerSustain AQ-C₁₈ column (250 mm×4.6 mm, 5 μm) with gradient elution of[acetonitrile-anhydrous ethanol (80:20)]-0.1% phosphoric acid solution. The detection wavelength was set at 220 nm, column temperature was set at 30℃, the flow rate was set at 1.0 mL/min, and the injection volume was 10 μL. Results Magnoliatine, phellodendrine, jatrorrhizine, palmatine, berberine, sophocarpine, matrine, oxysophocarpine, sophoridine and oxymatrine was linear within the range of 2.69-134.50, 1.95-97.50, 0.63-31.50, 0.86-43.00, 11.95-597.50, 0.59-29.50, 6.08-304.00, 4.85-242.50, 1.66-83.00, and 19.79-989.50 μg/mL(r ≥ 0.999 3). The average recovery were 99.11%, 98.23%, 96.95%, 97.78%, 100.02%, 97.21%, 99.66%, 99.52%, 98.81%, and 100.08%, with RSD values of 1.04%, 1.23%, 1.37%, 1.65%, 0.70%, 1.28%, 0.65%, 0.81%, 1.11%, and 0.63%, respectively. Conclusion The established HPLC method can be used for the simultaneous determination of ten active components in Kangfuyan Capsules, which can be used as the quality control method for Kangfuyan Capsules.

R284

國家自然科學(xué)基金青年科學(xué)基金項目（81703357）