18色譜柱(100 mm×2.1 mm,1.7 μm);檢測器:DAD檢測器;流動相為乙腈–0.1%磷酸溶液,梯度洗脫;檢測波長:331 nm;體積流量:1.0 mL/min;柱溫:30℃;進樣體積:25 μL。結(jié)果 鹽酸小檗堿、蒼術(shù)素和蛇床子素分別在8.954~447.700、3.980~199.000、8.670~433.500 μg/mL具有良好的線性(r≥0.995);鹽酸小檗堿、蒼術(shù)素、蛇床子素平均回收率分別為99.69%、98.78%、99.38%,RSD值分別為1.9%、1.6%、1.5%。結(jié)論 本法具有快速、準確、簡便等特點,為進一步提高烏蛇止癢丸的質(zhì)量控制提供技術(shù)參考。;Objective To explore a method for determination of osthol, atractylodin, and berberine hydrochloride in Wushe Zhiyang Pills by UPLC. Methods The HPLC method was carried out on Waters Acquity Beh C18 column (100 mm×2.1 mm,1.7 μm). Detector was selected as DAD. The mobile phase was consisted of acetontrile-0.1% phosphoric acid solution with gradient elution. The detection wavelength was set at 331 nm. The flow rate was 1.0 mL/min, temperature of column was set at 30℃, and the volume of injection was 25 μL. Results Berberine hydrochloride, atractylodin, and osthole showed good linear relationships in concentration range 8.954-447.700 μg/mL, 3.980-199.000 μg/mL, and 8.670-433.500 μg/mL (r ≥ 0.995). Average recoveries of samples were 99.69%, 98.78%, and 99.38% with RSD values as 1.9%, 1.6%, and 1.5%, respectively. Conclusion This method is fast, accurate, and simple, which provides a technical reference for improving the quality control of Wushe Zhiyang Pills."/>

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首頁 > 過刊瀏覽>2021年第36卷第12期 >2021,36(12):2507-2510. DOI:10.7501/j.issn.1674-5515.2021.12.007
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UPLC-DAD法測定烏蛇止癢丸中蛇床子素、蒼術(shù)素和鹽酸小檗堿

Determination of osthol, atractylodin, and berberine hydrochloride in Wushe Zhiyang Pills by UPLC-DAD

發(fā)布日期:2021-12-22