18色譜柱（250 mm×4.6 mm，5 μm）；乙腈–0.1%甲酸為流動(dòng)相，梯度洗脫；體積流量1.0 mL/min；檢測波長分別為360 nm（0~24 min檢測槲皮素、山柰酚、木犀草素和芹菜素）、330 nm（24~60 min檢測大車前苷、毛蕊花糖苷、異毛蕊花糖苷和木通苯乙醇苷B）；柱溫30 ℃；進(jìn)樣量10 μL。采用SPSS 26.0統(tǒng)計(jì)軟件對12批復(fù)方地錦膠囊中8種成分測定結(jié)果進(jìn)行聚類分析和主成分分析。結(jié)果 8種成分線性范圍良好（0.999 3≤r≤0.999 9）；平均加樣回收率97.24%~100.08%；聚類分析和主成分分析結(jié)果一致，12批復(fù)方地錦膠囊聚為3類，主成分1~4是影響復(fù)方地錦膠囊質(zhì)量評價(jià)的主要因子。結(jié)論 所建立的HPLC定量控制方法可用于復(fù)方地錦膠囊中多指標(biāo)成分的綜合質(zhì)量評價(jià)。;Objective To establish an HPLC method for determination of quercetin, kaempferol, luteolin, apigenin, plantamajoside, verbascoside, isoacteoside, and calceolarioside B in Compound Dijin Capsules, and the content determination results were comprehensive quality evaluated by chemometrics analysis.Methods The separation was carried out on Acclaim 120 C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.1% formic acid with gradient elution. The detection wavelength was set at 360 nm for quercetin, kaempferol, luteolin and apigenin, and 330 nm for plantamajoside, verbascoside, isoacteoside and calceolarioside B. The cluster analysis and principal component analysis were performed on the quantitative determination results of 8 components in 12 batches of Compound Dijin Capsules by SPSS 26.0 statistical software.Results The results showed that the eight components had good linear relationship (0.999 3≤ r ≤0.999 9), whose average recoveries were 97.24% — 100.08%. The results of cluster analysis and principal component analysis were consistent. 12 Batches of Compound Dijin Capsules samples were clustered into 3 categories, and the principal component 1 — 4 was the main factor affecting the quality evaluation of Compound Dijin Capsules.Conclusion The established HPLC method can be used for multi-index comprehensive quality evaluations of components in Compound Dijin Capsules."/>

醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

首頁 > 過刊瀏覽>2022年第37卷第1期 >2022,37(1):58-63. DOI:10.7501/j.issn.1674-5515.2022.01.008
上一篇 | 下一篇

復(fù)方地錦膠囊中8種成分的HPLC法測定及其化學(xué)計(jì)量學(xué)綜合評價(jià)

Determination of eight components in Compound Dijin Capsules by HPLC and comprehensive evaluation of chemometrics

發(fā)布日期：2022-01-24
  • 摘要
  • 圖/表
  • 訪問統(tǒng)計(jì)
  • PDF預(yù)覽
  • 參考文獻(xiàn)
  • 相似文獻(xiàn)
  • 引證文獻(xiàn)
  • 附件
    [關(guān)鍵詞]
    [摘要]
    目的 建立復(fù)方地錦膠囊中槲皮素、山柰酚、木犀草素、芹菜素、大車前苷、毛蕊花糖苷、異毛蕊花糖苷和木通苯乙醇苷B的高效液相色譜測定方法，并結(jié)合化學(xué)計(jì)量學(xué)綜合評價(jià)對其進(jìn)行綜合質(zhì)量評價(jià)。方法 采用Acclaim 120 C18色譜柱（250 mm×4.6 mm，5 μm）；乙腈–0.1%甲酸為流動(dòng)相，梯度洗脫；體積流量1.0 mL/min；檢測波長分別為360 nm（0~24 min檢測槲皮素、山柰酚、木犀草素和芹菜素）、330 nm（24~60 min檢測大車前苷、毛蕊花糖苷、異毛蕊花糖苷和木通苯乙醇苷B）；柱溫30 ℃；進(jìn)樣量10 μL。采用SPSS 26.0統(tǒng)計(jì)軟件對12批復(fù)方地錦膠囊中8種成分測定結(jié)果進(jìn)行聚類分析和主成分分析。結(jié)果 8種成分線性范圍良好（0.999 3≤r≤0.999 9）；平均加樣回收率97.24%~100.08%；聚類分析和主成分分析結(jié)果一致，12批復(fù)方地錦膠囊聚為3類，主成分1~4是影響復(fù)方地錦膠囊質(zhì)量評價(jià)的主要因子。結(jié)論 所建立的HPLC定量控制方法可用于復(fù)方地錦膠囊中多指標(biāo)成分的綜合質(zhì)量評價(jià)。
    [Key word]
    [Abstract]
    Objective To establish an HPLC method for determination of quercetin, kaempferol, luteolin, apigenin, plantamajoside, verbascoside, isoacteoside, and calceolarioside B in Compound Dijin Capsules, and the content determination results were comprehensive quality evaluated by chemometrics analysis.Methods The separation was carried out on Acclaim 120 C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.1% formic acid with gradient elution. The detection wavelength was set at 360 nm for quercetin, kaempferol, luteolin and apigenin, and 330 nm for plantamajoside, verbascoside, isoacteoside and calceolarioside B. The cluster analysis and principal component analysis were performed on the quantitative determination results of 8 components in 12 batches of Compound Dijin Capsules by SPSS 26.0 statistical software.Results The results showed that the eight components had good linear relationship (0.999 3≤ r ≤0.999 9), whose average recoveries were 97.24% — 100.08%. The results of cluster analysis and principal component analysis were consistent. 12 Batches of Compound Dijin Capsules samples were clustered into 3 categories, and the principal component 1 — 4 was the main factor affecting the quality evaluation of Compound Dijin Capsules.Conclusion The established HPLC method can be used for multi-index comprehensive quality evaluations of components in Compound Dijin Capsules.
    [中圖分類號]
    R286.02
    [基金項(xiàng)目]
    國家自然科學(xué)基金青年科學(xué)基金項(xiàng)目（81703357）