目的 采用超高效液相色譜-四級桿-靜電場軌道阱聯(lián)用技術（UPLC-Q-Exactive-Orbitrap-MS）辨別分析白花蛇舌草注射液中化學成分。方法 采用Waters Acquity UPLC HSS T3 C₁₈色譜柱（100 mm×2.1 mm，1.8 μm），以0.1%甲酸水溶液-乙腈為流動相，梯度洗脫，柱溫30℃，體積流量0.30 mL/min，樣品室溫度10℃，進樣體積10 μL。采用電噴霧離子源（ESI），正、負離子模式下采集數據。通過NIST質譜數據庫檢索，結合保留時間、分子離子、主要碎片，并根據文獻資料，推測其化學成分。結果 白花蛇舌草注射液中共鑒定出22個化合物，其中包括黃酮類7個、有機酸類7個，糖類3個、萜類2個、嘌呤2個、丁香脂素和酚苷各1個。結論 建立的方法能快速、準確、高效鑒定白花蛇舌草注射液中多種化學成分，為闡明白花蛇舌草的藥效物質基礎提供參考。

Objective To analyze chemical constituents in Baihuasheshecao Injection by UPLC-Q-Exactive-Orbitrap-MS. Methods The separation was performed on Waters Acquity UPLC HSS T3 C₁₈ column (100 mm×2.1 mm, 1.8 μm). The mobile phase was 0.1% formic acid water-acetonitrile for gradient elution. The column temperature was 30℃, flow rate was 0.30 mL/min, the sample room temperature was 10℃, and the injection volume was 10 μL. Electron spray ionization (ESI) source was adopted with both positive and negative ion modes. The chemical constituents were analyzed based on NIST mass spectrometry library, the retention time, and fragment ion peak of the compounds, as well as comparison with literature data. Results 22 Chemical constituents in Baihuasheshecao Injection were identified, including 7 flavonoids, 7 organic acids, 3 sugars, 2 terpenoids, 2 purines, 1 eugenin, and 1 phenolic glycoside. Conclusion The established method can quickly, accurately, and efficiently identify various components in Baihuasheshecao Injection, and provided a reference for elucidating the pharmacodynamic material basis of Hedyotis diffusa.

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廣西衛(wèi)健委自籌經費科研課題（Z20181009）；廣西中藥局自籌經費科研課題（GZZC2019034）