目的 建立高效液相色譜-一測(cè)多評(píng)法（HPLC-QAMS）測(cè)定肝爽顆粒中虎杖苷、白藜蘆醇、氧化芍藥苷、芍藥苷和柴胡皂苷a、b₁、d的方法。方法 采用Agilent SB-C₁₈色譜柱（250 mm×4.6 mm，5μm）；流動(dòng)相為乙腈-0.1%磷酸溶液，梯度洗脫；檢測(cè)波長(zhǎng)分別為306 nm（0~17 min，測(cè)定虎杖苷和白藜蘆醇）、230 nm（17~28 min，測(cè)定氧化芍藥苷和芍藥苷）、210 nm（28~55 min，測(cè)定柴胡皂苷a、b₁、d）；體積流量1.0 mL/min；柱溫30℃；進(jìn)樣量10 μL。以芍藥苷為內(nèi)參物，建立其他6個(gè)成分的相對(duì)校正因子（RCF）計(jì)算。結(jié)果 虎杖苷、白藜蘆醇、氧化芍藥苷、芍藥苷和柴胡皂苷a、b₁、d分別在6.37~159.25、4.91~122.75，1.88~47.00、7.99~199.75、0.56~14.00、2.19~54.75、2.87~71.75μg/mL線性關(guān)系良好，平均回收率分別為100.08%、98.95%、97.87%、99.52%、96.97%、98.88%、99.07%，RSD值分別為0.72%、1.1%、1.4%、0.99%、1.2%、1.2%、0.84%。肝爽顆粒中各成分的一測(cè)多評(píng)法所得結(jié)果與外標(biāo)法結(jié)果無(wú)明顯差異。結(jié)論 所建立的HPLC-QAMS法可用于肝爽顆粒中虎杖苷、白藜蘆醇、氧化芍藥苷、芍藥苷和柴胡皂苷a、b₁、d的同時(shí)測(cè)定。

Objective To establish an high performance liquid chromatography-quantitative analysis of multi-component by single marker (HPLC-QAMS) method for determination of polydatin, resveratrol, oxypaeoniflorin, paeoniflorin, saikosaponin a, saikosaponin b₁, and saikosaponin d in Ganshuang Granules. Methods Agilent SB-C₁₈ (250 mm×4.6 mm,5 μm) was used as the column with acetonitrile-0.1% phosphoric acid as the mobile phase by gradient elution. The detection wavelength were 306 nm for polydatin and resveratrol, 230 nm for oxypaeoniflorin and paeoniflorin, and 210 nm for saikosaponin a, saikosaponin b₁, and saikosaponin d. The flow rate was 1.0 mL/min, the column temperature was 30℃, and the injection volume was 10 μL. With paeoniflorin as the internal reference, the relative correction factors (RCF) of the other six constituents were calculated, and the content were determined. Results Polydatin, resveratrol, oxypaeoniflorin, paeoniflorin, saikosaponin a, saikosaponin b₁, and saikosaponin d showed good linear relationships within the ranges of 6.37-159.25, 4.91-122.75, 1.88-47.00, 7.99-199.75, 0.56-14.00, 2.19-54.75, 2.87-71.75 μg/mL, whose average recoveries (RSDs) were 100.08% (0.72%), 98.95% (1.1%), 97.87% (1.4%), 99.52% (0.99%), 96.97% (1.2%), 98.88% (1.2%), and 99.07% (0.84%), respectively. There was no significant difference on components contents between the quantitative results of HPLC-QAMS and those of external standard method (ESM). Conclusion HPLC-QAMS method can be used for the simultaneous determination of polydatin, resveratrol, oxypaeoniflorin, paeoniflorin, saikosaponin a, saikosaponin b₁, and saikosaponin d in Ganshuang Granules.

R286.02