目的 探討環(huán)泊酚通過Wnt/β-catenin信號(hào)通路對帕金森病模型大鼠的神經(jīng)保護(hù)作用。方法 采用隨機(jī)抓取的方法將30只大鼠分為對照組、模型組和環(huán)泊酚組,每組各10只。模型組和環(huán)泊酚組復(fù)制帕金森病模型,對照組注射等量葵花油。環(huán)泊酚組大鼠ig環(huán)泊酚溶液2 mg/kg,對照組和模型組大鼠ig等量生理鹽水,1次/d,連續(xù)14 d。電鏡下觀察中腦神經(jīng)細(xì)胞的超微結(jié)構(gòu),免疫組化染色觀察黑質(zhì)酪氨酸羥化酶(TH)陽性表達(dá),Western blotting法檢測Wnt和β-catenin蛋白表達(dá)。結(jié)果 與模型組比較,環(huán)泊酚組神經(jīng)細(xì)胞和線粒體數(shù)量增多,TH陽性表達(dá)率升高,Wnt5a、磷酸化β-catenin和核內(nèi)β-catenin蛋白表達(dá)量下降,差異均有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論 中腦神經(jīng)細(xì)胞減少、線粒體功能障礙、TH陽性表達(dá)下降以及Wnt/β-catenin信號(hào)通路異常激活可能與帕金森病的發(fā)生有關(guān),而環(huán)泊酚可通過改善神經(jīng)細(xì)胞和線粒體功能、增加TH表達(dá)以及抑制Wnt/β-catenin信號(hào)通路激活對帕金森病發(fā)揮神經(jīng)保護(hù)作用。

Objective To investigate the neuroprotective effect of cyclophenol for Parkinson's disease rats through Wnt/ β-catenin signaling pathway. Methods 30 Rats were divided into control group, model group, and cyclophenol group according to random grab method,10 rats in each group. The Parkinson's disease model was established in model group and cyclophenol group, and the control group was injected with the same amount of sunflower oil. Then the cyclophenol group was ig administered with cyclophenol solution (2 mg/kg), and the control group and model group were ig administered with the same amount of normal saline, once daily for 14 d. The ultrastructure of midbrain neurons was observed under electron microscopy, positive expression of tyrosine hydroxylase (TH) in substantia nigra was stained by immunohistochemistry, and Wnt and β-catenin proteins expression were detected by Western blotting method. Results Compared with the model group, the number of nerve cells and mitochondria in the cyclophenol group were increased. The positive expression rate of TH in cyclophenol group was higher than that in model group, and Wnt5a, phosphorylate β-catenin, and intranuclear β-catenin proteins were decreased, with statistically significant differences (P < 0.05). Conclusion Neurocytopenia, mitochondrial dysfunction, less TH positive expression, and abnormal activation of Wnt/β-catenin signaling pathway in midbrain may be related to the occurrence of Parkinson's disease, and cyclophenol can improve the function of nerve cells and mitochondria, increase TH expression and inhibit activation of Wnt/β-catenin signaling pathway, which plays a neuroprotective role in Parkinson's disease.

R965

河南省高等學(xué)校重點(diǎn)科研項(xiàng)目計(jì)劃（20B320002，19A320021）