目的 采用高效液相色譜-二極管陣列檢測(cè)器(HPLC-DAD)法測(cè)定腸胃舒膠囊中木香烴內(nèi)酯、去氫木香內(nèi)酯、沒(méi)食子酸和血竭素。方法 采用Waters Sunfire C18色譜柱(250 mm×4.6 mm, 5μm); DAD;流動(dòng)相:乙腈- 0.2%磷酸溶液,梯度洗脫;檢測(cè)波長(zhǎng):木香烴內(nèi)酯(225 nm)、去氫木香內(nèi)酯(225 nm)、沒(méi)食子酸(272 nm)、血竭素(440 nm);體積流量1.0 mL/min;柱溫25℃;進(jìn)樣體積10μL。結(jié)果 木香烴內(nèi)酯、去氫木香內(nèi)酯、沒(méi)食子酸、血竭素分別在0.999~99.900、0.995~99.500、0.275~27.500、0.250~25.000μg/mL線(xiàn)性良好,平均回收率分別為99.2%、99.5%、99.1%、99.9%, RSD值分別為1.1%、1.6%、1.3%、1.7%。結(jié)論 本法具有簡(jiǎn)便、準(zhǔn)確等特點(diǎn),可以用于腸胃舒膠囊的質(zhì)量控制。

Objective To establish an HPLC-DAD method for determination of aucklan lactone, dehydrocarbactone, gallic acid, and draconisin in Changweishu Capsules.Methods Waters Sunfire C18 column (250 mm×4.6 mm， 5 μm) was used with acetonitrile - 0.2% phosphoric acid solution as the mobile phase by gradient elution. DAD detector was used for quantitative detection. The detection wavelength were 225 nm (aucklan lactone), 225 nm (dehydrocarbactone), 272 nm (gallic acid), and 440 nm (draconis). The flow rate was 1.0 mL/min, the column temperature was 25 ℃, and the injection volume was 10 μL.Results The linearity of lignan lactone, dehydrocarbactone, gallic acid and draconisin were good in the range of 0.999 — 99.900 μg/mL, 0.995 — 99.500 μg/mL, 0.275 — 27.500 μg/mL, and 0.250 — 25.000 μg/mL. The average recoveries were 99.2%, 99.5%, 99.1% and 99.9%, with RSD 2.4%, 1.6%, 2.1%, and 1.8%, respectively..Conclusion The method is simple and accurate, which can be used for the quality control of Changweishu Capsules.

R286.02