目的 觀察隱丹參酮對慢性不可預(yù)見應(yīng)激（CUMS）聯(lián)合脂多糖（LPS）所致抑郁小鼠氧化應(yīng)激和炎癥反應(yīng)的影響。方法 60只清潔級ICR小鼠隨機(jī)分為對照組、模型組、帕羅西汀組（20 mg/kg）及隱丹參酮低、中、高劑量（10、20、40 mg/kg）組，每組10只。采用CUMS＋LPS應(yīng)激刺激14 d建立抑郁模型（除對照組），同時(shí)ig相應(yīng)藥物或生理鹽水，1次/d，連續(xù)14 d。通過體質(zhì)量增量、糖水偏好指數(shù)、懸尾實(shí)驗(yàn)及新奇環(huán)境攝食測試評價(jià)抑郁行為，并測定各組血清超氧化物歧化酶（SOD）、過氧化氫酶（CAT）、谷胱甘肽過氧化物酶（GSH-Px）、丙二醛（MDA），海馬和皮質(zhì)白細(xì)胞介素（IL）-6、IL-1β、腫瘤壞死因子（TNF）-α變化情況。結(jié)果 隱丹參酮20、40 mg/kg組體質(zhì)量增量及SOD、CAT、GSH-Px酶活性高于模型組，不動時(shí)間短于模型組（P＜0.05、0.01），海馬IL-6、海馬和皮質(zhì)IL-1β含量低于模型組（P＜0.05、0.01）。各治療組糖水偏好指數(shù)高于模型組、攝食潛伏期短于模型組（P＜0.05、0.01），血清MDA、皮質(zhì)TNF-α含量低于模型組（P＜0.05、0.01）。隱丹參酮40 mg/kg組皮質(zhì)IL-6、海馬TNF-α含量低于模型組（P＜0.01）。結(jié)論 隱丹參酮對CUMS聯(lián)合LPS致小鼠抑郁癥狀有改善作用，其機(jī)制可能與抑制過強(qiáng)的氧化應(yīng)激反應(yīng)與神經(jīng)炎癥反應(yīng)，并減輕神經(jīng)元損傷有關(guān)。

Objective To observe the effects of cryptotanshinone on oxidative stress and inflammatory response in depressed mice induced by chronic unpredictable stress (CUMS) combined with lipopolysaccharide (LPS). Methods Sixty clean grade ICR mice were randomly divided into control group, model group, paroxetine group (20 mg/kg), cryptotanshinone low-dose, medium-dose, and high-dose (10, 20, 40 mg/kg), with 10 mice in each group. Depression model was established by CUMS + LPS stress stimulation for 14 d. At the same time, relevant drugs or normal saline were given intragastrically, once daily, for consecutive 14 d. Depressive behaviors were evaluated by body weight gain, sugar water preference index, tail suspension test, and novel environmental feeding test, and serum superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were determined. Changes of interleukin (IL-6), IL-1β, tumor necrosis factor (TNF) -α in hippocampus and cortex were determined. Results Body weight increment, SOD, CAT, and GSH-Px enzyme activities in 20 and 40 mg/kg cryptotanshinone groups were higher than those in model group, and immobility time was shorter than that in model group (P < 0.05, 0.01). The contents of IL-6 in hippocampus, and IL-1β in hippocampus and cortex were lower than those in model group (P < 0.05, 0.01). The preference index of sugar water in treatment groups was higher than that in model group, and the incubation period of ingestion was shorter than that in model group (P < 0.05, 0.01). The contents of MDA in serum and TNF-α in cortex were lower than those in model group (P < 0.05, 0.01). The contents of IL-6 in cortex and TNF-α in hippocampus of cryptotanshinone 40 mg/kg group were lower than those of model group (P < 0.01). Conclusion Cryptotanshinone can improve the depressive symptoms of CUMS + LPS mice, and the mechanism may be related to the inhibition of excessive oxidative stress response, and neuroinflammatory response, and the alleviation of neuronal injury.

R965

福建省中青年教師教育科研項(xiàng)目(JAT171155);泉州市指導(dǎo)性科技計(jì)劃項(xiàng)目(2021N130S)