目的 通過(guò)構(gòu)建支氣管肺炎SD大鼠實(shí)驗(yàn)?zāi)Ｐ停骄考t景天苷對(duì)支氣管肺炎大鼠核因子紅細(xì)胞相關(guān)因子2（Nrf2）/ Kelch樣環(huán)氧氯丙烷相關(guān)蛋白（Keap1）通路及氣道炎癥的影響。方法 將SD大鼠隨機(jī)分為對(duì)照組、模型組及紅景天苷低、高劑量（30、60 mg/kg）組和鴉膽子苦醇組，紅景天苷＋鴉膽子苦醇組，每組10只。紅景天苷組大鼠分別ip 30、60 mg/kg紅景天苷，鴉膽子苦醇組大鼠ip 100 mg/mL鴉膽子苦醇，紅景天苷＋鴉膽子苦醇組ip 60 mg/kg紅景天苷和100 mg/mL鴉膽子苦醇。對(duì)照組使用同等劑量生理鹽水。取各組大鼠肺泡灌洗液，對(duì)灌洗液中嗜酸性粒細(xì)胞、中性粒細(xì)胞和淋巴細(xì)胞數(shù)量進(jìn)行分類和計(jì)數(shù)，ELISA法檢測(cè)肺泡灌洗液中白細(xì)胞介素-1β（IL-1β）、白細(xì)胞介素-13（IL-13）、白細(xì)胞介素-18（IL-18）水平。蘇木精–伊紅（HE）染色法觀測(cè)各組大鼠肺組織形態(tài)，Western blotting法檢測(cè)大鼠肺組織IL-1β、誘導(dǎo)型一氧化氮合酶（iNOS）及Nrf2/Keap1信號(hào)通道相關(guān)蛋白表達(dá)水平。結(jié)果 與模型組相比，紅景天苷30、60 mg/kg組大鼠肺泡灌洗液中嗜酸性粒細(xì)胞、中性粒細(xì)胞和淋巴細(xì)胞數(shù)量，IL-1β、IL-13、IL-18水平及肺組織IL-1β、iNOS、Keap1蛋白表達(dá)、炎癥評(píng)分顯著降低，肺組織Nrf2蛋白表達(dá)水平顯著升高（P＜0.05）；與紅景天苷60 mg/kg組相比，紅景天苷＋鴉膽子苦醇組大鼠肺泡灌洗液中嗜酸性粒細(xì)胞、中性粒細(xì)胞和淋巴細(xì)胞數(shù)量，肺泡灌洗液IL-1β、IL-13、IL-18水平，肺組織IL-1β、iNOS、Keap1蛋白表達(dá)水平，炎癥評(píng)分顯著升高，肺組織Nrf2蛋白表達(dá)水平顯著降低（P＜0.05）。結(jié)論 紅景天苷可能通過(guò)激活Nrf2/Keap1信號(hào)通道緩解支氣管肺炎炎癥。

Objective To explore the effects of salidroside on Nrf2/Keap1 pathway and airway inflammation in rats with bronchopneumonia by constructing an experimental model of SD rats.Methods SD rats were randomly divided into control group,model group,salidroside low and high dose (30 and 60 mg/kg) groups,Brusatol group,salidroside+Brusatol group,with 10 rats in each group.Salidroside group rats ip 30 and 60 mg/kg salidroside,Brusatol group rats ip 100 mg/mL Brusatol,salidroside+Brusatol group ip 60 mg/kg salidroside and 100 mg/mL Brusatol,respectively.The control group received the same dose of normal saline.The numbers of eosinophils,neutrophils,and lymphocytes in the lavage fluid were classified and counted.The levels of IL-1β,IL-13,and IL-18 in the lavage fluid were detected by ELISA.The morphology of lung tissue was observed by hematoxylin and eosin (HE) staining.The expression levels of IL-1β,iNOS,and Nrf2/Keap1 signaling channel related proteins in lung tissue were detected by Western blotting.Results Compared with the model group,the numbers of eosinophils,neutrophils,and lymphocytes in the alveolar lavage fluid,the levels of IL-1β,IL-13 and IL-18,the protein expressions of IL-1β,iNOS and Keap1 in lung tissue,and the inflammation score of the salidroside 30 and 60 mg/kg groups were significantly decreased,the protein expression level of Nrf2 was significantly increased (P<0.05).Compared with salidroside 60 mg/kg group,the number of eosinophils,neutrophils,and lymphocytes in alveolar lavage fluid,the levels of IL-1β,IL-13 and IL-18 in alveolar lavage fluid,the protein expression levels of IL-1β,iNOS and Keap1 in lung tissue,and inflammation score of rats in salidroside+Brusatol group were significantly increased,the protein expression level of Nrf2 was significantly decreased (P<0.05).Conclusion Salidroside may alleviate the inflammation of bronchopneumonia by activating the Nrf2/Keap1 signal pathway.

R285.5

天津醫(yī)科大學(xué)2019年度醫(yī)院管理創(chuàng)新研究項(xiàng)目(2019YG07)