目的 建立了HPLC-DAD波長切換法測定通絡(luò)開痹片中5-O-甲基維斯阿米醇苷、士的寧、馬錢子堿和杯莧甾酮的分析方法。方法 Purospher ODS C₁₈色譜柱（250mm×4.6mm，5μm）；以乙腈–0.5%磷酸溶液為流動相，梯度洗脫；測定波長：260nm（士的寧和馬錢子堿）、243nm（杯莧甾酮）、254nm（5-O-甲基維斯阿米醇苷）；體積流量：1.0mL/min；柱溫：30℃；進樣體積：10μL。結(jié)果 杯莧甾酮、馬錢子堿、士的寧和5-O-甲基維斯阿米醇苷分別在0.0584～5.8400、0.1180～11.8000、0.0949～9.9000、0.0238～2.3800μg/mL具有良好的線性，平均回收率分別為100.51%、99.59%、98.75%、100.38%，RSD值分別為1.6%、1.1%、1.7%、1.6%。結(jié)論 本法具有前處理簡單、分析時間短、檢測結(jié)果準確等優(yōu)點，適用于通絡(luò)開痹片的質(zhì)量控制。

Objective To establish an HPLC-DAD wavelength switching method for determination of 5-O-methylvisamitol glycoside, strychnine, brucine, and cyasterone in Tongluo Kaibi Tablets. Methods Purospher ODS C₁₈ column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was consisted of acetonitrile-0.5% phosphoric acid solution with gradient elution. The detection wavelengths were set at 260 nm for strychnine and brucine, 243 nm for cyasterone, and 254 nm for 5-O-methylvisamitol glycoside. The column temperature was 30℃, volume flow was 1.0 mL/min, and the injection volume was 10 μL. Results Cyasterone, brucine, strychnine, and 5-O-methylvisamitol glycoside showed good linear relationships in concentration range of 0.0584-5.8400, 0.1180-11.8000, 0.0949-9.9000, and 0.0238-2.3800 μg/mL. The average recoveries were 100.51%, 99.59%, 98.75%, and 100.38% with RSD values of 1.6%, 1.1%, 1.7%, and 1.6%, respectively. Conclusion The method has the advantages of simple pretreatment, short analysis time, and accurate detection result, which is suitable for quality control of Tongluo Kaibi Tablets.

R286.02