目的 采用多指標(biāo)成分、化學(xué)計(jì)量學(xué)聯(lián)合熵權(quán)優(yōu)劣解距離法綜合評價(jià)蘇木的質(zhì)量。方法 采用HPLC法測定不同產(chǎn)地蘇木中巴西蘇木素、蘇木精、原蘇木素A、(±)原蘇木素B、蘇木酮A、鷹嘴豆芽素A、異甘草素和蘇木查耳酮;對以上結(jié)果進(jìn)行聚類分析、主成分分析、正交偏最小二乘法-判別分析,尋找影響蘇木質(zhì)量的主要潛在標(biāo)志物;采用熵權(quán)優(yōu)劣解距離法對不同產(chǎn)區(qū)蘇木的質(zhì)量優(yōu)劣進(jìn)行評價(jià)。結(jié)果 18批蘇木聚為3類,不同產(chǎn)地蘇木質(zhì)量呈現(xiàn)一定的區(qū)域差異。蘇木精、巴西蘇木素、原蘇木素A對蘇木質(zhì)量差異影響較大,是影響蘇木質(zhì)量的主要潛在標(biāo)志物。廣西、廣東所產(chǎn)蘇木質(zhì)量最優(yōu),其次為四川、貴州和云南,福建地區(qū)產(chǎn)品質(zhì)量較差。結(jié)論 本實(shí)驗(yàn)建立了蘇木質(zhì)量綜合評價(jià)方法,操作便捷、可行性高、實(shí)用性強(qiáng),為更加科學(xué)、系統(tǒng)地控制蘇木質(zhì)量提供技術(shù)支撐。

Objective To comprehensively evaluate the quality of Sappan Lignum by multiple index, chemometrics, and EW- TOPSIS method. Methods HPLC method was used to simultaneously determine the contents of brazilin, hematoxylin, protosappanin A, (±) protosappanin B, sappanone A, biochanin A, isoliquiritigenin, and sappanchalcone in Sappan Lignum from different origins. The results were analyzed by clustering analysis (CA), principal component analysis (PCA), and orthogonal partial least squares- discriminant analysis (OPLS-DA) to look for the main potential markers affecting the quality of Sappan Lignum. Qualities of Sappan Lignum in different producing areas were evaluated by entropy TOPSIS (EW-TOPSIS) method. Results 18 Batches of Sappan Lignum could be clustered into 3 categories, and the quality of Sappan Lignum from different origins showed certain regional differences. Hematoxylin, brazilin, and protosappanin A had great influence on the quality difference of Sappan Lignum, and were the main potential markers affecting the quality of Sappan Lignum. The quality of Sappan Lignum from Guangxi and Guangdong Province was the best, followed by Sichuan, Guizhou and Yunnan Province, and that from Fujian Province was poor. Conclusion The established comprehensive evaluation method for the quality of Sappan Lignum is easy to operate, highly feasible, and practical, which can provide technical support for more scientific and systematic control of the quality of Sappan Lignum.

R286.02

北京市屬醫(yī)院科研培育計(jì)劃中醫(yī)項(xiàng)目(PZ2021005);北京市中醫(yī)藥管理局第二批中藥骨干人才培養(yǎng)項(xiàng)目(京中醫(yī)科字[2018]213號)