目的 建立測(cè)定芪桂痛風(fēng)片中新綠原酸、秦皮甲素、綠原酸、秦皮乙素、秦皮苷、秦皮素、4,5-O-二咖啡?？鼘幩帷⒚苫ㄜ盏腍PLC方法。方法 采用ACE Excel C₁₈-AR色譜柱（150 mm×4.6 mm，3 μm）；流動(dòng)相為0.1%甲酸水–乙腈，梯度洗脫；檢測(cè)波長(zhǎng)325 nm（新綠原酸、綠原酸、4,5-O-二咖啡?？鼘幩幔?、334 nm（秦皮甲素、秦皮乙素、秦皮苷、秦皮素、蒙花苷）；體積流量1 mL/min；柱溫30 ℃；進(jìn)樣量10 μL。結(jié)果 新綠原酸、秦皮甲素、綠原酸、秦皮乙素、秦皮苷、秦皮素、4,5-O-二咖啡?？鼘幩?、蒙花苷的線性范圍分別為1.86～59.45、26.21～838.80、8.01～256.47、3.60～115.45、17.79～569.18、1.93～61.76、3.67～117.51、2.27～72.76 μg/mL，線性關(guān)系良好（r＝1.000 0），平均回收率分別為95.56%、92.88%、95.49%、92.15%、93.03%、96.03%、99.90%、95.42%，RSD值分別為3.40%、3.11%、3.25%、3.17%、3.10%、3.52%、3.40%、3.34%。結(jié)論 建立的芪桂痛風(fēng)片中8種成分HPLC定量方法簡(jiǎn)便、快速、準(zhǔn)確，可以用于評(píng)價(jià)芪桂痛風(fēng)片的質(zhì)量。

Objective To establish an HPLC method for determination of neochlorogenic acid, esculin, chlorogenic acid, esculetin, fraxin, fraxetin, 4,5-O-dicaffeoylquinic acid, and linarin in Qigui Tongfeng Tablets. Methods The separation was carried out on Ace Excel C₁₈-AR column (150 mm × 4.6 mm，3 μm). The mobile phase was acetonitrile -0.1% formic acid with gradient elution. The detection wavelength was set at 325 nm (neochlorogenic acid, chlorogenic acid, and 4,5-O-dicaffeoylquinic acid) and 334 nm (esculin, esculetin, fraxin, fraxetin, and linarin). The flow rate was 1.0 mL/min, temperature of column was set at 30 ℃, and the volume of injection was 10 μL. Results Neochlorogenic acid, esculin, chlorogenic acid, esculetin, fraxin, fraxetin, 4,5-O-dicaffeoylquinic acid, and linarin had good linear relationships in concentration range of 1.86 — 59.45, 26.21 — 838.80, 8.01 — 256.47, 3.60 — 115.45, 17.79 — 569.18, 1.93 — 61.76, 3.67 — 117.51, and 2.27 — 72.76 μg/mL (r＝1.000 0), and their average recoveries were 95.56%, 92.88%, 95.49%, 92.15%, 93.03%, 96.03%, 99.90%, and 95.42% with RSD values as 3.40%, 3.11%, 3.25%, 3.17%, 3.10%, 3.52%, 3.40%, and 3.34%, respectively. Conclusion The established HPLC quantitative method for eight components in Qigui Tongfeng Tablets is simple, rapid, and accurate, and can be used to evaluate the quality of Qigui Tongfeng Tablets.

R286.02

國(guó)家中醫(yī)藥管理局岐黃學(xué)者項(xiàng)目（2021）