目的 探究炔雌醇對(duì)絕經(jīng)后骨質(zhì)疏松癥模型小鼠骨質(zhì)量的影響。方法 將30只SPF級(jí)C57雌性小鼠隨機(jī)分為3組，每組10只，分別為假手術(shù)組、模型組和炔雌醇組，炔雌醇組每天ig炔雌醇100 μg/kg，假手術(shù)組和模型組均ig給予等體積蒸餾水，給藥12周后取材，分別觀察臟器系數(shù)、主要臟器和骨組織病理學(xué)切片，分析Micro CT、生物力學(xué)和血清生化指標(biāo)。結(jié)果 與模型組相比，炔雌醇組小鼠體質(zhì)量顯著下降（P＜0.05、0.01）。炔雌醇組的臟器系數(shù)除了子宮與模型組和假手術(shù)組有顯著性差異，其余均沒(méi)有顯著性差異，主要臟器未發(fā)現(xiàn)明顯的病變。骨組織脫鈣切片顯示，與模型組相比，炔雌醇組骨小梁結(jié)構(gòu)致密，脂肪細(xì)胞較少。與模型組相比，炔雌醇組生物力學(xué)的最大載荷和彈性模量得到了明顯的提高（P＜0.05、0.01）。與模型組比較，炔雌醇組小鼠的骨小梁骨密度（Tb.BMD）、骨小梁數(shù)（Tb.N）、骨小梁厚度（Tb.Th）和骨體積分?jǐn)?shù)（Tb.BV/TV）水平均顯著增加，骨小梁分離度（Tb.Sp）數(shù)值顯著減少（P＜0.01）。與模型組比較，炔雌醇組血清骨鈣素（OCN）和I型前膠原N端前肽（PINP）的平均水平顯著升高；抗酒石酸酸性磷酸酶5b（TRACP-5b）和I型膠原交聯(lián)C端肽（CTX-I）水平顯著降低（P＜0.01）。結(jié)論 炔雌醇能提高絕經(jīng)后骨質(zhì)疏松癥模型小鼠骨質(zhì)量同時(shí)對(duì)小鼠主要臟器沒(méi)有損害。

Objective To investigate the effects of ethinylestradiol on bone quality in postmenopausal osteoporosis mice. Methods Thirty SPF C57 female mice were randomly divided into 3 groups with 10 mice in each group: sham operation group, model group, and ethinylestradiol group. Ethinylestradiol group was given 100 μg/kg ethinylestradiol intragastrically every day, sham operation group and model group were given equal volume of distilled water intragastrically. Samples were taken 12 weeks after administration, and organ coefficients, major organs and bone histopathological sections were observed, respectively, and Micro CT, biomechanical and serum biochemical indexes were analyzed. Results Compared with model group, the body weight of ethinylestradiol group was significantly decreased (P < 0.05, 0.01). The organ coefficients of ethinylestradiol group mice showed no significant differences except uterus, model group, and sham operation group, and no obvious lesions were found in the main organs. Compared with the model group, the bone trabecular structure in the ethinylestradiol group was dense and there were fewer fat cells. Compared with the model group, the maximum biomechanical load and elastic modulus of ethinylestradiol group were significantly increased (P < 0.05, 0.01). Compared with model group, the Tb.BMD, Tb.N, Tb.Th, and Tb.BV/TV levels of ethinylestradiol group were significantly increased, while the Tb.Sp value was significantly decreased (P < 0.01). Compared with model group, the mean serum OCN and PINP levels in ethinylestradiol group were significantly increased, TRACP-5b and CTX-I levels were significantly decreased (P < 0.01). Conclusion Ethinylestradiol can improve the bone quality of postmenopausal osteoporosis mice without damaging the major organs.

R977.1

國(guó)家自然科學(xué)基金面上項(xiàng)目（81770879）；甘肅省青年科技基金計(jì)劃項(xiàng)目（20JR5RA589）；聯(lián)勤保障部隊(duì)第九四〇醫(yī)院實(shí)驗(yàn)室培育項(xiàng)目（2021yxky081）