目的 探究丹參多酚酸對腦梗死大鼠神經(jīng)血管微循環(huán)及缺血側(cè)血流的影響。方法 將大鼠隨機分為假手術(shù)組、模型組、尼莫地平10 mg/kg組和丹參多酚酸低、高劑量組（10、25 mg/kg）。制備大鼠中動脈缺血模型，通過神經(jīng)功能評分、2，3，5-三苯基氯化四氮唑染色以及采用激光散斑血流成像檢測腦血流量，鑒定模型構(gòu)建是否成功。各組大鼠按照分組進行給藥，尼莫地平組ig尼莫地平10 mg/kg，丹參多酚酸組ip 10、25 mg/kg注射用丹參多酚酸，連續(xù)治療7 d，1次/d。取腦組織分別進行2,3,5-三苯基氯化四氮唑染色染色評估腦梗死體積，伊文思藍含量測定評估血、腦、脊液屏障結(jié)構(gòu)完整性，免疫熒光染色測量血小板內(nèi)皮細胞黏附分子（CD31）表達。采用Western blotting實驗檢測血管內(nèi)皮生長因子（VEGF）、血管生成素-1（Ang-1）、磷酸甘油醛脫氫酶（GAPDH）蛋白表達。結(jié)果 與模型組相比，丹參多酚酸25 mg/kg組神經(jīng)功能評分、腦梗死體積占比明顯減低，腦血流量明顯增加（P＜0.05）；且缺血側(cè)CD31相對表達明顯減少；伊文思藍含量以及VEGF與Ang1蛋白表達明顯增加（P＜0.05）。結(jié)論 丹參多酚酸能夠明顯促進神經(jīng)血管微循環(huán)重構(gòu)，加速缺血區(qū)血流恢復(fù)，最終減輕缺血性卒中損傷作用，其機制可能與VEGF與Ang1蛋白表達增加相關(guān)。

Objective To investigate the effects of salvianolic acids on neural angiogenesis and cerebral blood flow in rats with ischemic stroke. Methods Rats were randomly divided into sham operation group, model group, nimodipine 10 mg/kg group, and salvianolic acid low and high-dose groups (10 and 25 mg/kg). The rat model of middle artery ischemia was prepared, and the cerebral blood flow was detected by nerve function score, 2,3,5-triphenyltetrazolium chloride staining and laser speckle flow imaging to determine whether the model was successfully constructed. The rats in each group were given medication according to the groups, nimodipine group ig Nimodipine Tablets 10 mg/kg, salvianolic acid group ip 10, 25 mg/kg Salvianolic acid for injection, continuous treatment for 7 d, once daily. 2,3,5-triphenyltetrazazole chloride staining was performed on the brain tissues to evaluate the infarct volume, evans blue content was determined to evaluate the structural integrity of the blood, brain, and spinal fluid barrier, and the expression of platelet endothelial cell adhesion molecule (CD31) was measured by immunofluorescence staining. The protein expressions of vascular endothelial growth factor (VEGF), Ang-1, and phosphoglyceraldehyde dehydrogenase (GAPDH) were detected by Western blotting assay. Results Compared with model group, 25 mg/kg salvianolic acid group significantly decreased the neurological function score and cerebral infarction volume ratio, and significantly increased cerebral blood flow (P < 0.05). The relative expression of CD31 in ischemic side was significantly decreased, Evans blue content and the expression of VEGF and Ang1 protein were significantly increased (P < 0.05). Conclusion Salvianolic acids can significantly promote neurovascular microcirculation remodeling, accelerate the recovery of blood flow in the ischemic area, and ultimately reduce the injury of ischemic stroke. The mechanism may be related to the increase of VEGF and Ang1 protein expression.

R971

海南省衛(wèi)生健康行業(yè)科研項目（22A200042）