目的 對深綠卷柏和江南卷柏的質量控制和抗氧化活性進行比較。方法 對深綠卷柏和江南卷柏進行性狀鑒別、顯微鑒別和薄層色譜鑒別，采用HPLC法測定其中的穗花杉雙黃酮，測定石油醚、醋酸乙酯和正丁醇部位的DPPH自由基清除率、ABTS⁺·清除能力。結果 深綠卷柏和江南卷柏的性狀、顯微特征均有差異，薄層色譜中具有相同黃綠色熒光斑點，也有差異性；江南卷柏中穗花杉雙黃酮的質量分數(shù)高于深綠卷柏；不同萃取部位的抗氧化活性的能力高低為醋酸乙酯＞正丁醇＞石油醚，且江南卷柏醋酸乙酯部位抗氧化活性優(yōu)于深綠卷柏。結論 深綠卷柏和江南卷柏有顯著差異，江南卷柏要比深綠卷柏抗氧化活性更好。

Objective To compare quality control and antioxidant activity between S. doederleinii and S. moellendorffii. Methods The character identification, microscopic identification, and TLC identification of S. doederleinii and S. moellendorffii were carried out. The contents of amentoflavone were determined by HPLC method, and the DPPH free radical scavenging rate and ABTS⁺· scavenging capacity of petroleum ether, ethyl acetate and n-butanol fractions were measured. Results S. doederleinii and S. moellendorffii had differences in characters and microscopic characteristics, and TLC results of them had the same chartreuse fluorescent spots, but also have differences. The contents of amentoflavone in S. moellendorffii were higher than those in S. doederleinii. The antioxidant activities of different fractions were as follows: ethyl acetate > n-butanol > petroleum ether, and the antioxidant activity of the ethyl acetate fraction of S. moellendorffii was better than that of S. doederleinii. Conclusion There is a significant difference between S. doederleinii and S. moellendorffii, and S. moellendorffii has more antioxidant activities than S. doederleinii.

R286.02

河南省藥品監(jiān)督管理局科技計劃項目（2020DB057）