目的 考察金蓮花湯中葛根素、牡荊素、迷迭香酸和咖啡酸的腸吸收特性，確定金蓮花湯中活性成分。方法 采用HPLC法測定腸吸收樣品中咖啡酸、葛根素、牡荊素、迷迭香酸，以大鼠腸外翻吸收實(shí)驗(yàn)和Caco-2細(xì)胞模型轉(zhuǎn)運(yùn)實(shí)驗(yàn)計(jì)算各成分的累積吸收量、吸收速率常數(shù)和表觀滲透系數(shù)。結(jié)果 隨著金蓮花湯的質(zhì)量濃度增加，咖啡酸、葛根素、牡荊素、迷迭香酸的吸收速率常數(shù)呈線性關(guān)系增加，符合零級吸收速率，且吸收率均小于1.4%，在腸道中吸收效果普遍較差，其中咖啡酸的吸收較為明顯。各成分的表觀滲透系數(shù)值大小為迷迭香酸>咖啡酸>葛根素>牡荊素。結(jié)論 金蓮花湯中葛根素、牡荊素、迷迭香酸和咖啡酸均可在腸道中被吸收，吸收方式為被動(dòng)擴(kuò)散，咖啡酸和迷迭香酸可能是金蓮花湯中發(fā)揮藥效的活性物質(zhì)，葛根素和牡荊素可能是前藥。

Objective To investigate intestinal absorption of puerarin, vitexin, rosmarinic acid, and caffeic acid in Jinlianhua Decoction, and to determine the active components. Methods The contents of of puerarin, vitexin, rosmarinic acid, and caffeic acid were detected by HPLC method. Cumulative absorption amount, absorption rate constant, and permeability coefficient (P_app) of each component were calculated using everted intestinal sac model and Caco-2 cell monolayer model. Results As the concentration of Jinlianhua Decoction increases, the absorption rate constants of caffeic acid, puerarin, vitexin, and rosmarinic acid increase linearly with a zero-order reaction. Their absorption rates were below 1.4%, the absorption effect in the intestine was generally poor, and the caffeic acid was the highest. The P_app values were rosmarinic acid > caffeic acid > puerarin > vitexin. Conclusion Puerarin, vitexin, rosmarinic acid, and caffeic acid in Jinlianhua Decoction can be absorbed in the intestine with passive diffusion. Caffeic acid and rosmarinic acid may be active substances in Jinlianhua Decoction, while puerarin and vitexin may be prodrugs.

R286.02

中央引導(dǎo)地方科技發(fā)展資金項(xiàng)目（206Z2502G）