目的 建立獨(dú)活寄生丸的指紋圖譜,并采用化學(xué)計(jì)量學(xué)進(jìn)行評(píng)價(jià)。方法 采用HPLC法建立獨(dú)活寄生丸的指紋圖譜和相似度評(píng)價(jià),以聚類(lèi)分析、主成分分析、正交偏最小二乘判別分析法分析數(shù)據(jù)。結(jié)果 建立了獨(dú)活寄生丸HPLC特征指紋圖譜,共確定32個(gè)共有峰,指認(rèn)6個(gè)成分龍膽苦苷、川芎嗪、芝麻脂素、蛇床子素、細(xì)辛脂素、二氫歐山芹醇當(dāng)歸酸酯。15批獨(dú)活寄生丸與對(duì)照?qǐng)D譜的相似度均大于0.900。15批獨(dú)活寄生丸樣品可分為2類(lèi),S1、S4、S6聚為一類(lèi),其余樣品為另一類(lèi)。9個(gè)主成分因子可作為獨(dú)活寄生丸的評(píng)價(jià)指標(biāo)。對(duì)模型分類(lèi)貢獻(xiàn)較大的3個(gè)變量,分別為共有峰4、17、2。結(jié)論 該方法可用于獨(dú)活寄生丸的質(zhì)量評(píng)價(jià),為獨(dú)活寄生丸的質(zhì)量標(biāo)準(zhǔn)研究提供依據(jù)。

Objective To establish the fingerprint of Duhuo Jisheng Pills, and to evaluate it with stoichiometry.Methods Fingerprints of Duhuo Jisheng Pills were established by HPLC method, and similarity was evaluated. Cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis were used to analyze the data.Results The characteristic fingerprint of Duhuo Jisheng Pills was established and 32 common peaks were established. Six components gentiopicroside, ligustrazine, sesamin, osthol, asarone, and columbianadin were identified. The similarity between the fingerprints of Duhuo Jisheng Pills of 15 batches and the control fingerprints were all above 0.900. Duhuo Jisheng Pills of 15 batches could be divided into two categories, S1, S4, and S6 as one category, while the other samples belong to another category. Nine principal component factors can be used as evaluation indicators for Duhuo Jisheng Pills. The three variables that contribute significantly to model classification were the common peaks 4, 17, and 2.Conclusion The method can be used to evaluate the quality of Duhuo Jisheng Pills, which can provide a basis for the study of quality standards for Duhuo Jisheng Pills.

R286.02

國(guó)家自然科學(xué)基金資助項(xiàng)目(82074271);中央支持地方高校改革發(fā)展資金(2020YQ05)