目的 優(yōu)化樹脂柱色譜法脫除銀杏葉提取物中總銀杏酸的最佳工藝。方法 通過單因素和響應(yīng)面法實(shí)驗(yàn),以體積流量、洗脫劑乙醇體積分?jǐn)?shù)、洗脫液接收體積為考察因素,以銀杏葉提取物中總銀杏酸去除率、總黃酮轉(zhuǎn)移率和提取物收率的綜合評分為指標(biāo),優(yōu)化樹脂柱色譜法脫除總銀杏酸最佳工藝。結(jié)果 最佳工藝參數(shù)為體積流量1.2 BV/h,洗脫劑乙醇體積分?jǐn)?shù)70%,洗脫液接收體積3 BV。結(jié)論 本工藝穩(wěn)定、可行、重復(fù)性好,可作為銀杏葉提取物樹脂柱色譜脫酸工藝。

Objective To optimize process for removing total ginkgolic acids in Ginkgo biloba extract by resin column chromatography. Methods Single factor and response surface method experiment was used, the flow rate, ethanol concentration of eluent, and collection volume of eluent was as factors, and the removal rate of total ginkgolic acids, transfer rate of total flavonoids, and yield of Ginkgo biloba extract were comprehensively evaluated. The process of removing total ginkgolic acids by resin column chromatography was optimized. Results The optimal process parameters were the flow rate of 1.2 BV/h, the ethanol concentration of eluent of 70%, and the eluent collection volume of 3 BV. Conclusion The process is stable, feasible, and has good repeatability, and can be used as resin column chromatography deacidification process for Ginkgo biloba extract.

R284.2

山東省重點(diǎn)研發(fā)計(jì)劃(重大科技創(chuàng)新工程)項(xiàng)目(2021CXGC010508)