目的 調(diào)查分析南方醫(yī)科大學(xué)附屬深圳婦幼保健院分離真菌的分布特點(diǎn)和藥物敏感性情況,為婦幼真菌感染疾病的臨床診治、耐藥性監(jiān)測(cè)和流行病學(xué)研究提供參考。方法 回顧分析南方醫(yī)科大學(xué)附屬深圳婦幼保健院2018年1月-2022年12月標(biāo)本采集、真菌鑒定和抗真菌藥敏試驗(yàn)的結(jié)果數(shù)據(jù)。結(jié)果 共分離真菌3 350株,前2位分別為白色念珠菌1 542株、光滑念珠菌223株。陽性標(biāo)本以陰道分泌物/宮頸分泌物為主。婦科和產(chǎn)科分離到的真菌最多。白色念珠菌對(duì)伊曲康唑、伏立康唑的5年耐藥率分別為9.58%、4.12%,對(duì)兩性霉素B和5-氟胞嘧啶的5年敏感率分別為99.79%、98.01%,對(duì)氟康唑的歷年敏感率有逐漸升高的態(tài)勢(shì)。光滑念珠菌、近平滑念珠菌和熱帶念珠菌對(duì)兩性霉素B及5-氟胞嘧啶的敏感率均為100.00%?？巳崮钪榫鷮?duì)兩性霉素B和伏立康唑100.00%敏感。結(jié)論 南方醫(yī)科大學(xué)附屬深圳婦幼保健院分離真菌以念珠菌屬為主,其中白色念珠菌最多。陰道分泌物/宮頸分泌物是主要真菌陽性分離標(biāo)本。白色念珠菌對(duì)兩性霉素B的耐藥率最低、敏感率最高,對(duì)伊曲康唑的耐藥率最高、敏感率最低。光滑念珠菌、近平滑念珠菌、熱帶念珠菌和克柔念珠菌對(duì)兩性霉素B和5-氟胞嘧啶均無耐藥性。光滑念珠菌對(duì)伊曲康唑耐藥率最高、敏感率最低。

Objective To investigate and analyze the distribution characteristics and drug susceptibility of fungi isolated from Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, and to provide reference for clinical diagnosis and treatment of maternal and child fungal infectious diseases, antifungal resistance surveillance and epidemiological research. Methods The resulting data of specimen collection, fungus identification and antifungal susceptibility testing in Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, from January 2018 to December 2022 were collected for descriptive retrospective analysis. Results 3 350 Strains of non-repetitive fungi were isolated. The top two were 1 542 strains of Candida albicans and 223 strains of Candida glabrata, respectively. The fungal positive specimens were mainly vaginal secretions/cervical secretions. Among the permanent departments, the most fungi were isolated from gynaecology and obstetrics department. The five-year drug resistance rates of Candida albicans to itraconazole and voriconazole were 9.58% and 4.12%, respectively, the five-year susceptibility rates to amphotericin B and 5-fluorocytosine were 99.79% and 98.01%, respectively, and the susceptibility rate to fluconazole over the years was gradually increasing. The susceptibility rates of Candida glabrata, Candida parapsilosis, and Candida tropicalis to amphotericin B and 5-fluorocytosine were all 100.00%. Candida krusei was 100.00% sensitive to amphotericin B and voriconazole. Conclusions From 2018 to 2022, the main fungi isolated from Shenzhen Maternal and Child Health Hospital affiliated to Southern Medical University were Candida, among which Candida albicans was the most common. Vaginal secretion/cervical secretion is the main fungal positive isolation specimen. Candida albicans had the lowest resistance rate and the highest sensitivity rate to amphotericin B, and the highest resistance rate and the lowest sensitivity rate to itraconazole. Candida glabrata, Candida parapsilosis, and Candida tropicalis, and Candida krusei have no resistance to amphotericin B and 5-fluorocytosine. Candida glabrata has the highest resistance rate to itraconazole and the lowest sensitivity rate.

R978.5

國家自然科學(xué)基金資助項(xiàng)目(81702087)；廣東省基礎(chǔ)與應(yīng)用基礎(chǔ)研究基金項(xiàng)目(2020A1515010590)；廣東省醫(yī)學(xué)科學(xué)技術(shù)研究基金項(xiàng)目(A2020097)；深圳市科技計(jì)劃項(xiàng)目(JCYJ20220530155213031)