目的 研究六神丸對小鼠口腔潰瘍的療效及可能的作用機制。方法 將32只C57BL/6JGpt級小鼠隨機分成對照組、模型組、六神丸組、桂林西瓜霜組，每組8只。于小鼠口腔左側(cè)頰部黏膜上使用氫氧化鈉片狀晶體灼燒構(gòu)建口腔潰瘍模型。對口腔潰瘍造模成功的小鼠分別外敷涂抹給予六神丸和桂林西瓜霜粉末0.375 g，連續(xù)5 d。觀察小鼠口腔潰瘍的恢復狀態(tài)，用ELISA試劑盒檢測血清白細胞介素（IL）-6、IL-8水平。取口腔潰瘍部位組織，將潰瘍組織制備勻漿，用二喹啉酸（BCA）法測定總蛋白濃度，并用Western blotting方法檢測細胞外調(diào)節(jié)蛋白激酶（ERK）磷酸化水平。結(jié)果 與模型組比較，六神丸組小鼠口腔潰瘍部位組織恢復情況較好，IL-6、IL-8水平顯著降低（P＜0.05），局部組織中ERK磷酸化水平降低。結(jié)論 六神丸對氫氧化鈉灼燒的口腔潰瘍小鼠模型具有一定治療作用，且可能的機制是通過降低血液中IL-6和IL-8水平，進而導致局部組織中ERK磷酸化水平降低改善病理狀態(tài)。

Objective To study the treatment effect of Liushen Pills for oral ulcer in mice. Methods Thirty-two C57BL/6JGpt mice were randomly divided into control group, model group, Liushen Pills group, and Guilin Watermelon Cream group, each grop had 8 mice. The oral ulcer model was constructed by burning the left buccal mucosa of the oral cavity of mice with sodium hydroxide flake crystal. Mice with successfully constructed oral ulcer were treated with Liushen Pills and Guilin Watermelon Cream for 5 d. To observe the recovery state of oral ulcer in mice, serum levels of IL-6 and IL-8 were detected by ELISA kit. The ulcerated tissues of oral cavity were taken and homogenized. The total protein concentration was determined by BCA method, and the phosphorylation level of extracellular regulated protein kinase (ERK) was detected by Western blotting method. Results Compared with model group, the oral ulcer tissues of mice in Liushen Pills group and Guilin Watermelon Cream group recovered better, the levels of IL-6 and IL-8 were significantly decreased (P < 0.05), and the phosphorylation level of ERK in local tissues was decreased. Conclusion Liushen Pills have a certain therapeutic effect on the NaOH burning oral ulcer mouse model, and the action was performed by decreasing the IL-6 and IL-8 in blood circulation and thus down-regulate the ERK1/2 phsphorlation level in local tissue.

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