目的 闡明利血平對脊髓小腦共濟失調(diào)3型（SCA3）細胞模型的潛在作用。方法 通過核質(zhì)分離、免疫熒光、Western blotting、微孔過濾和細胞活性等實驗探索利血平對SCA3發(fā)病過程中ataxin-3核質(zhì)轉(zhuǎn)運、ataxin-3可溶性蛋白水平、蛋白包涵體、細胞活性等一系列病理變化的影響。結(jié)果 利血平對ataxin-3核質(zhì)轉(zhuǎn)運和可溶性蛋白水平?jīng)]有顯著性影響，但是增加了ataxin-3形成的包涵體數(shù)量，并且增加了細胞毒性。結(jié)論 利血平通過增加包涵體數(shù)量導致SCA3細胞活性減弱，為老藥新用發(fā)現(xiàn)提供了理論依據(jù)。

Objective To clarify the potential effect of reserpine on spinocerebellar ataxia type 3 (SCA3) cell model. Methods The effects of reserpine on ataxin-3 nuclear and cytoplasmic transport, ataxin-3 soluble protein level, protein inclusion body and cell viability during the pathogenesis of SCA3 were investigated by nucleocytoplasmic separation, immunofluorescence, Western blotting, filter trap and CCK-8 methods. Results Reserpine had no significant effect on the nucleocytoplasmic transport and soluble protein level of ataxin-3, but increased the number of inclusion bodies formed by ataxin-3 and increased cytotoxicity. Conclusion Reserpine decreases the SCA3 cell viability by increasing the number of inclusion bodies, which provides a theoretical basis for the new discovery of old drugs.

R966

國家自然科學基金資助項目（82202067，32270530）；陜西省自然科學基礎(chǔ)研究計劃資助項目（2020JM-620）；西安市科技計劃創(chuàng)新基金“文理專項”項目（2020KJWL04）；西安文理學院博士啟動金（06005026）；西安文理學院院長基金（YZJJ202104）；國家級大學生創(chuàng)新創(chuàng)業(yè)項目（S202011080012，S202311080020）；省級大學生創(chuàng)新創(chuàng)業(yè)項目（S202011080058，S202111080043，S202111080031）