目的 考察炮制過(guò)程中茅根炭飲片UPLC指紋圖譜和色度值的差異。方法 采用UPLC法建立白茅根、茅根炭的指紋圖譜，并采用分光測(cè)色儀測(cè)定其色度值（L*、a*、b*），以各類(lèi)分析方法分析茅根炭炮制過(guò)程中飲片指紋圖譜與色度值的相關(guān)性。結(jié)果 隨著炮制時(shí)間的延長(zhǎng)，茅根炭較白茅根指紋圖譜的相似度逐漸降低；峰1、5-羥甲基糠醛、峰11、4-香豆酸單位峰面積先增大后減小，其余共有峰單位峰面積均呈減小趨勢(shì)；L*、b*值整體上逐漸減小，而△E*逐漸增大。5-羥甲基糠醛、峰3、峰9峰面積與色度值間具有極顯著性或顯著性。主成分分析共提取2個(gè)主成分，累積方差貢獻(xiàn)率為88.870%。聚類(lèi)分析結(jié)果顯示，炮制時(shí)間2～6 min聚為第1類(lèi)，炮制時(shí)間8～16 min聚為第2類(lèi)，炮制時(shí)間18～30 min聚為第3類(lèi)。正交偏最小二乘法判別分析結(jié)果顯示，峰1、a*、b*、峰3、L*、峰12、E*、5-羥甲基糠醛是茅根炭飲片炮制過(guò)程中發(fā)生質(zhì)量變化的主要指標(biāo)。結(jié)論 不同炮制時(shí)間茅根炭飲片的色度值與UPLC指紋圖譜密切相關(guān)，可為茅根炭炮制過(guò)程中監(jiān)測(cè)飲片質(zhì)量提供參考。

Objective To investigate the difference between UPLC fingerprint and chromaticity value of carbonized Imperatae Rhizoma of different processing time. Methods UPLC method was used to establish fingerprint, and spectrophotometric colorimeter was used to determine the chromaticity value (L*, a*, b*). And the correlation difference between UPLC fingerprint and chromaticity value of carbonized Imperatae Rhizoma during processing was analyzed using various analytical methods. Results With the prolonging of processing time, the similarity of carbonized Imperatae Rhizoma and Imperatae Rhizoma gradually decreased. The unit areas of peak 1, 5-hydroxymethylfurfural (5-HMF), peak 11, and p-coumaric acid first increased and then decreased, while the other common peaks showed a decreasing trend. The values of L* and b* gradually decreased, while △E* gradually increased. 5-HMF, peak 3, and peak 9 had extremely significant or significant correlation between peak area and chromaticity value. Two principal components were extracted by principal component analysis, and the cumulative variance contribution rate was 88.870%. The results of cluster analysis showed that the samples processed for 2 — 6, 8 — 16, and 18 — 30 min were clustered into three classes. The results of orthogonal partial least squares discriminant analysis showed that peak 1, a*, b*, peak 3, L*, peak twelve, E*, and 5-HMF were the main indexes of quality change of carbonized Imperatae Rhizoma during processing. Conclusion The chromaticity values of carbonized Imperatae Rhizoma at different processing times is closely related to UPLC fingerprint, which can provide reference for monitoring the quality of carbonized Imperatae Rhizoma.

R284

工業(yè)和信息化部2022年產(chǎn)業(yè)技術(shù)基礎(chǔ)公共服務(wù)平臺(tái)（2022-230-221）