目的 建立HPLC法同時(shí)測(cè)定糖痹通絡(luò)顆粒中原兒茶酸、羥基紅花黃色素A、對(duì)香豆酸、阿魏酸、β-蛻皮激素、絡(luò)石苷。方法 采用ACE 5 C₁₈-AR色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相乙腈–0.1%磷酸水溶液，梯度洗脫；檢測(cè)波長260 nm（原兒茶酸、β-蛻皮激素）、280 nm（對(duì)香豆酸、阿魏酸、絡(luò)石苷）、360 nm（羥基紅花黃色素A）；體積流量1 mL/min；柱溫40 ℃；進(jìn)樣量5 μL。結(jié)果 原兒茶酸、羥基紅花黃色素A、對(duì)香豆酸、阿魏酸、β-蛻皮激素、絡(luò)石苷在各自線性范圍內(nèi)線性關(guān)系良好（r≥0.999 5），平均加樣回收率分別為103.63%、96.93%、103.18%、97.09%、102.38%、100.08%，RSD值分別為1.01%、0.88%、1.27%、0.90%、1.35%、1.05%。結(jié)論 該方法簡單準(zhǔn)確、穩(wěn)定可靠、重復(fù)性好，可用于糖痹通絡(luò)顆粒的質(zhì)量控制。

Objective To establish an HPLC method for the simultaneous determination of protocatechuic acid, hydroxysafflor yellow A, p-coumaric acid, erulic acid, β-ecdysterone, and tracheloside in Tangbi Tongluo Granules. Methods The analysis was performed on ACE 5 C₁₈-AR column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.1% phosphoric acid solution with gradient elution. The detection wavelength was set at 260 nm (protocatechuic acid and β-ecdysterone), 280 nm (p-coumaric acid, ferulic acid, and tracheloside), 360 nm (hydroxysafflor yellow A). The flow rate was 1.0 mL/min, temperature of column was set at 40 ℃, and the volume of injection was 5 μL. Results Protocatechuic acid, hydroxysafflor yellow A, p-coumaric acid, erulic acid, β-ecdysterone, and tracheloside showed good linear relationships within their respective linear ranges (r ≥ 0.999 5), whose average recoveries were 103.63%, 96.93%, 103.18%, 97.09%, 102.38%, and 100.08% with RSD values of 1.01%, 0.88%, 1.27%, 0.90%, 1.35%, and 1.05%, respectively. Conclusion The method is simple accurate, stable reliable, and repeatable, which can be used to control the quality of Tangbi Tongluo Granules.

R286.02

國家自然科學(xué)基金資助項(xiàng)目（82174293，81774117）；國家自然科學(xué)基金青年科學(xué)基金資助項(xiàng)目（82004286）；江蘇省中醫(yī)藥科技發(fā)展計(jì)劃項(xiàng)目（ZD202208）；江蘇省中醫(yī)藥科技發(fā)展計(jì)劃項(xiàng)目（ZT202206）