目的 基于網(wǎng)絡(luò)藥理學(xué)及分子對接技術(shù)探討青風(fēng)藤治療痛風(fēng)性關(guān)節(jié)炎的作用機制。方法 運用中藥系統(tǒng)藥理學(xué)數(shù)據(jù)庫與分析平臺（TCMSP），獲取青風(fēng)藤的活性成分，并通過SwissTarget Prediction數(shù)據(jù)庫預(yù)測其靶點。使用GeneCards、OMIM、DisGenet、TTD數(shù)據(jù)庫獲取與痛風(fēng)性關(guān)節(jié)炎相關(guān)的靶點，通過維恩圖找到兩者的共同靶點，并將其輸入STRING平臺和Cytoscape 3.9.1構(gòu)建蛋白質(zhì)相互作用（PPI）網(wǎng)絡(luò)圖。利用DAVID數(shù)據(jù)庫對共同靶點進(jìn)行基因本體（GO）生物學(xué)富集分析和京都基因與基因組百科全書（KEGG）通路富集分析。借助AutoDockTools分子對接和可視化處理。結(jié)果 獲得青風(fēng)藤6種有效活性成分，藥物與疾病靶點交集后獲得84個關(guān)鍵靶點，PPI顯示核心靶點有前列腺素內(nèi)過氧化物合酶2（PTGS2）、過氧化物酶體增殖物激活受體G（PPARG）、轉(zhuǎn)錄因子AP-1（JUN）、Toll樣受體4（TLR4）、一氧化氮合酶2（NOS2）等。KEGG通路分析，可能涉及了癌癥通路、腫瘤壞死因子（TNF）、Th17細(xì)胞分化、核因子κB（NF-κB）通路、脂質(zhì)和動脈粥樣硬化通路等。分子對接結(jié)果顯示，青風(fēng)藤的主要活性成分青藤堿、β-谷甾醇、拉茲馬寧堿、烏心石環(huán)氧內(nèi)脂、千金藤啶堿與關(guān)鍵蛋白PPARG、NOS2、PTGS2、TLR4表現(xiàn)出良好的結(jié)合能力。結(jié)論 青風(fēng)藤具有多成分、多靶點特性，通過多途徑來緩解炎癥反應(yīng)，對治療痛風(fēng)急性關(guān)節(jié)炎的發(fā)作具有潛在的療效。

Objective To explore the mechanism of Sinomenii Caulis in treating gouty arthritis based on network pharmacology and molecular docking techniques. Methods To obtain the effective active ingredients of Sinomenii Caulis and predict their targets through the TCMSP and SwissTarget Prediction database. Gouty arthritis related targets were obtained from GeneCards, OMIM, DisGenet, and TTD databases. The intersection targets of both sets were identified using a Venn diagram, and these were input into the STRING platform and Cytoscape 3.9.1 to construct a PPI network. The common targets were subjected to GO biological enrichment analysis and KEGG pathway enrichment analysis using the DAVID database. Molecular docking and visualization processing were performed using AutoDockTools. Results Six effective active ingredients of Sinomenii Caulis were obtained. After the intersection of drug and disease targets, 84 key targets were identified. The PPI network revealed core targets including PTGS2, PPARG, JUN, TLR4, NOS2, etc. KEGG pathway analysis suggested involvement in cancer pathways, TNF signaling, Th17 cell differentiation, NF-κB pathway, lipid metabolism, and atherosclerosis pathways, among others. Molecular docking results demonstrated favorable binding capabilities of Sinomenii Caulis main active components sinomenine, β-sitosterol, 16-epi-isositsirikine, michelenolide, stepholidine with key proteins PPARG, NOS2, PTGS2, and TLR4. Conclusion Sinomenii Caulis exhibits a multi-component, multi-target characteristic, alleviating inflammation through multiple pathways, indicating potential efficacy in treating acute attacks of gouty arthritis.

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天津市醫(yī)學(xué)重點學(xué)科（?？疲┙ㄔO(shè)項目（TJYXZDXK-032A）