目的 研究兒瀉康貼膜對功能性腹痛幼齡大鼠的影響，并探討其療效機制。方法 將出生1 d的SD大鼠隨機分為對照組和模型組，采用母嬰分離聯(lián)合冰水應激的方法建立功能性腹痛大鼠內(nèi)臟高敏感模型。選取造模成功的大鼠隨機分為模型組、丁桂兒臍貼組，兒瀉康貼膜（1/24、1/12、1/6貼）組。各組大鼠腹部貼劑給藥，連續(xù)7 d，對照組、模型組腹部給予空白模擬貼。觀察給藥后大鼠糞便Bristol分級評分、糞便含水率及腹壁撤退反射（AWR）評分結果；采用酶聯(lián)免疫吸附實驗（ELISA）法檢測血清和結腸組織中5-羥色胺（5-HT）、P物質(zhì)（SP）、腫瘤壞死因子-α（TNF-α）的含量；采用實時熒光定量聚合酶鏈式反應（RT-qPCR）測定結腸組織中囊性纖維化跨膜轉(zhuǎn)運調(diào)節(jié)體（CFTR）、促腎上腺皮質(zhì)激素釋放因子（CRF）mRNA表達水平；Western blotting法檢測CFTR、CRF蛋白表達水平。結果 與模型組相比，兒瀉康貼膜各劑量組大鼠糞便Bristol評分明顯降低，糞便含水量顯著減少，不同注水體積下的AWR評分均顯著降低，血清和結腸組織中致痛致瀉相關因子5-HT、SP、TNF-α的表達均得到顯著抑制，結腸組織中CFTR和CRF mRNA和蛋白的相對表達量均顯著降低（P＜0.05、0.01、0.001）。結論 兒瀉康貼膜對功能性腹痛大鼠有顯著的治療效果，其通過調(diào)節(jié)血清和結腸組織中腹痛腹瀉相關因子的含量以及結腸組織中相關蛋白的表達以顯著降低大鼠糞便Bristol評分和糞便含水量以及大鼠的內(nèi)臟敏感性。

Objective To observe effects of Erxiekang Plaster on functional abdominal pain juvenile rats, and explore its mechanism. Methods The 1-day-old SD rats were randomly divided into control group and model group. The functional abdominal pain rat models with visceral hypersensitivity were established by the method of combination of maternal separation and ice water stress. The rats with successful modeling were randomly divided into model group, Dingguier Umbilical Paste group, and Erxiekang Plaster (1/24, 1/12, 1/6) group. Each group of rats was given abdominal patches for 7 consecutive days, while the control group and model group were given blank simulated patches on the abdomen. Scores of Bristol classification and water content of the fecal pellets and abdominal withdrawal reflex (AWR) of rats in all the groups were observed after treatment. ELISA was used to detect the changes of 5-HT, SP and TNF-α in serum and colon tissue. The mRNA expression levels of CFTR and CRF in colonic tissues were detected by RT-PCR. The protein expression levels of both were detected by Western blotting. Results Compared with the model group, the Bristol score and fecal water content of rats significantly decreased after treatment with Erxiekang Plaster, and the AWR score significantly decreased under different water injection volumes. The expression levels of 5-HT, SP, and TNF-α in serum and colon tissue were significantly decreased. The CFTR and CRF mRNA and protein expression significantly down-regulated. Conclusion Erxiekang Plaster has a significant therapeutic effect on functional abdominal pain in rats. It can significantly reduce the Bristol score and fecal water content, as well as visceral sensitivity of rats by regulating the content of abdominal pain and diarrhea related factors in serum and colon tissue, as well as the expression of related proteins in colon tissue.

R285.5

安徽省科技重大專項項目（202303a0702007）