目的 觀察鼻咽清毒顆粒逆轉鼻咽癌細胞順鉑耐藥的作用效應，并探討其作用機制。方法 采用MTS法檢測系列質量濃度鼻咽清毒顆粒對人鼻咽癌CNE-2、人鼻咽癌順鉑耐藥CNE2/DDP細胞增殖的抑制作用；計算鼻咽清毒顆粒對CNE-2、CNE2/DDP細胞的耐順鉑半數抑制濃度（IC₅₀）的影響；流式細胞技術測定CNE2/DDP的細胞凋亡情況；采用Western blotting法檢測CNE2/DDP細胞中銅轉運蛋白和抗凋亡蛋白的表達。結果 CNE2/DDP細胞的順鉑耐藥指數為RI值為3.03；與順鉑組比較，鼻咽清毒顆粒0.1、1、10 mg/mL組分別與系列質量濃度的順鉑聯用，對CNE-2細胞順鉑IC₅₀分別降低12.19%、46.37%、63.81%；CNE2/DDP細胞順鉑IC₅₀分別降低61.75%、70.42%、83.82%。流式細胞技術檢測結果表明，與順鉑組比，順鉑＋鼻咽清毒顆粒0.1、1、10 mg/mL組CNE2-DDP晚期凋亡或壞死細胞增加（P＜0.01）；Western blotting法檢測結果表明，與順鉑組比較，順鉑＋鼻咽清毒顆粒0.1、1、10 mg/mL組銅特異性轉運蛋白（CTR1）蛋白的表達均顯著升高，B細胞淋巴瘤2（Bcl-2）、骨髓細胞白血病序列1（Mcl-1）的表達顯著降低（P＜0.05、0.01）。結論 鼻咽清毒顆粒具有增強鼻咽癌細胞順鉑敏感性、逆轉CNE2/DDP細胞順鉑耐藥作用，其作用機制可能與增加CTR1蛋白表達，降低Bcl-2、Mcl-1蛋白表達有關。

Objective To observe the effect of Biyan Qingdu Granules on reversing cisplatin resistance in nasopharyngeal carcinoma cells, and to explore its mechanism. Methods To detect the inhibitory effect of Biyan Qingdu Granules on the proliferation of CNE-2 and CNE2/DDP cells by MTS method. To calculate the effect of Biyan Qingdu Granules on cisplatin inhibiting IC₅₀ in CNE-2 and CNE2/DDP cells. The apoptosis of CNE2/DDP cells was determined by flow cytometry. The expressions of copper transporter and anti-apoptotic protein in CNE2/DDP cells were detected by Western Blotting. Results The cisplatin resistance index of CNE2/DDP cells was 3.03. Compared with cisplatin, the IC₅₀ of cisplatin in CNE-2 cells of 0.1, 1 and 10 mg/mL of Biyan Qingdu Granules was decreased by 12.19%, 46.37% and 63.81%, respectively. Cisplatin IC₅₀ in CNE2/DDP cells decreased by 61.75%, 70.42% and 83.82%, respectively. The results of flow cytometry showed that compared with cisplatin group, CNE2/DDP in Biyan Qingdu Granules 0.1, 1, 10 mg/mL groups increased late apoptosis or necrosis cells (P < 0.01). Western Blotting test results showed that compared with cisplatin group, the expression of copper specific transport protein (CTR1) in cisplatin + Biyan Qingdu Granules 0.1, 1 and 10 mg/mL groups was significantly increased. The expressions of B-cell lymphoma 2 (Bcl-2) and myeloid leukemia sequence 1 (Mcl-1) were significantly decreased (P < 0.05, 0.01). Conclusion Biyan Qingdu Granules can enhance the cisplatin sensitivity of nasopharyngeal carcinoma cells and reverse the cisplatin resistance of CNE2/DDP cells, and its mechanism may be related to increasing the expression of CTR1 protein and decreasing the expression of Bcl-2 and Mcl-1 protein.

R979.1

廣州市科技計劃項目（20212210006）