目的 建立仙茅HPLC多指標(biāo)成分定量-一測多評法，采用化學(xué)計(jì)量學(xué)、加權(quán)TOPSIS和灰色關(guān)聯(lián)度（GRA）融合模型評價不同產(chǎn)地仙茅質(zhì)量。方法 采用HPLC-一測多評法測定不同產(chǎn)地仙茅中仙茅苷乙、苔黑酚龍膽二糖苷、苔黑酚葡萄糖苷、苔黑素、2,6-二甲氧基苯甲酸、仙茅苷、仙茅木酚素、豆甾醇、胡蘿卜苷、β-谷甾醇，并檢測浸出物、總灰分和酸不溶性灰分，采用化學(xué)計(jì)量學(xué)分析數(shù)據(jù)，建立加權(quán)TOPSIS和GRA融合模型評價仙茅藥材質(zhì)量。結(jié)果 不同產(chǎn)地仙茅中各成分含量稍有波動；仙茅樣品大致分為3類；苔黑酚葡萄糖苷、仙茅苷、仙茅苷乙、苔黑酚龍膽二糖苷、仙茅木酚素和β-谷甾醇可作為不同產(chǎn)地仙茅的質(zhì)量差異因子。不同產(chǎn)地仙茅質(zhì)量存在顯著差異，其中編號S14的仙茅樣品的質(zhì)量最優(yōu)。結(jié)論 建立的方法簡單、檢測周期短，利于推廣，能較全面地評價仙茅的質(zhì)量。

Objective To establish an HPLC multi-index component analysis - QAMS method, and to evaluate the quality of Curculiginis Rhizoma from different regions by chemometrics, weighted TOPSIS, and GRA fusion model. Methods The contents of curculigoside B, orcinol gentiobioside, orcinol glucoside, orcinol, 2,6-dimethoxybenzoic acid, curculigoside, curlignan, stigmasterol, daucosterol, and β-sitosterol in Curculiginis Rhizoma were determined by HPLC - QAMS method, and the extract, total ash, and acid-insoluble ash were also detected. Data were analyzed by chemometrics and the weighted TOPSIS and GRA fusion model was established to evaluate the quality of Curculiginis Rhizoma. Results The content of various components in Curculiginis Rhizoma from different origins fluctuated slightly. Curculiginis Rhizoma were divided into 3 groups. Orcinol glucoside, curculigoside, curculigoside B, orcinol gentiobioside, curlignan and β-sitosterol could be used as quality differential factors for different origins of Curculiginis Rhizoma. There were significant differences in the quality of Curculiginis Rhizoma from different regions, among which sample S14 had the best quality. Conclusion The established method is simple with short measurement time, which is conducive to promotion and can comprehensively evaluate the quality of Curculiginis Rhizoma.

R286.02

河南省高等學(xué)校重點(diǎn)科研資助計(jì)劃項(xiàng)目(20A110024)