目的 建立HPLC波長切換法同時測定威喜丸中豬苓酮B、豬苓酮A、去氫土莫酸、豬苓酸C、去氫茯苓酸和茯苓酸的方法。方法 采用Agilent Eclipse XDB-C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相：乙腈–0.05%磷酸溶液，梯度洗脫；0～24.0 min在248 nm波長下檢測豬苓酮B和豬苓酮A，24.0～50.0 min在210 nm波長下檢測去氫土莫酸、豬苓酸C、去氫茯苓酸和茯苓酸；體積流量1.0 mL/min；柱溫30℃；進樣量為10 μL。結(jié)果 豬苓酮B、豬苓酮A、去氫土莫酸、豬苓酸C、去氫茯苓酸和茯苓酸分別在5.79～115.80、1.66～33.20、3.28～65.60、7.07～141.40、1.46～29.20、0.88～17.60μg/mL與峰面積線性關(guān)系良好；平均加樣回收率分別為98.75%、97.60%、98.23%、99.49%、97.10%、96.81%，RSD值分別為0.75%、1.66%、1.44%、0.90%、1.15%、1.38%。結(jié)論 本方法簡便、準確、重復(fù)性好，為威喜丸的質(zhì)量控制提供了實驗依據(jù)。

Objective To establish an HPLC with wavelength switching method for determination of polyporusterone B, polyporusterone A, dehydrotumulosic acid, polyporenic acid C, dehydropachymic acid, and pachymic acid in Weixi Pills. Methods The separation was carried out on Agilent Eclipse XDB-C₁₈ chromatography column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile - 0.05% phosphoric acid solution with gradient elution. The detection wavelengths were set at 248 nm in 0 - 24.0 min (determination of polyporusterone B and polyporusterone A) and 210 nm in 24.0 - 50.0 min (determination of dehydrotumulosic acid, polyporenic acid C, dehydropachymic acid and pachymic acid). The flow rate was 1.0 mL/min, temperature of column was set at 30℃, and volume of injection was 10 µL. Results The linear ranges of polyporusterone B, polyporusterone A, dehydrotumulosic acid, polyporenic acid C, dehydropachymic acid, and pachymic acid were 5.79 - 115.80, 1.66 - 33.20, 3.28 - 65.60, 7.07 - 141.40, 1.46 - 29.20, and 0.88 - 17.60 μg/mL, respectively. The average recoveries were 98.75%, 97.60%, 98.23%, 99.49%, 97.10%, and 96.81%, with RSD 0.75%, 1.66%, 1.44%, 0.90%, 1.15%, and 1.38%. Conclusion The method is simple, accurate and reproducible, and provides an experimental basis for the quality control of Weixi Pills.