目的：觀察NG701對(duì)泡沫細(xì)胞形成及CD36 mRNA表達(dá)的影響。方法：利用大鼠腹腔提取的巨噬細(xì)胞與氧化低密度脂蛋白共培養(yǎng)得到巨噬細(xì)胞源性泡沫細(xì)胞，加入不同濃度的NG701，觀察泡沫細(xì)胞內(nèi)膽固醇含量的變化，并利用RT-PCR法對(duì)CD36 mRNA表達(dá)情況進(jìn)行檢測(cè)。結(jié)果：NG701能夠顯著減少泡沫細(xì)胞內(nèi)總膽固醇和膽固醇酯的含量，降低CE/TC的值。并劑量依賴性的降低CD36 mRNA表達(dá)。結(jié)論：NG701能通過(guò)減少CD36表達(dá)抑制巨噬細(xì)胞源性泡沫細(xì)胞的形成。

Objective: To investigate the effect of NG701 on the formation of foam cell and the expression of CD36 mRNA. Methods: Macrophages were incubated with ox-LDL to establish the foam cell model. Different concentration of NG701 were given for intervention. The cellular lipid accumulation was examined by oil red staining. The cellular contents of total cholesterol (TC) and cholesterol ester (CE) were detected by enzymic methods. The levels of CD36 mRNA were determined by RT-PCR. Results: NG701 could significantly decrease the content of TC, CE, and CE/TC in foam cell in middle and high dose groups. RT-PCR assays showed that the expression of CD36 mRNA was down-regulated in a dose-dependent manner. Conclusion: NG701 could inhibit the formation of foam cell via down-regulating CD36 mRNA expression.