目的：建立參茸健脾膠囊中10-羥基-2-癸烯酸的HPLC測定方法。方法：采用Agilent 1100 DAD高效液相色譜儀，Agilent Extend C₁₈色譜柱（250 mm×4.6 mm，5 μm），乙腈-0.1％磷酸（23∶77）為流動相，檢測波長為207 nm，流速1 mL/min。結(jié)果：10-羥基-2-癸烯酸在0.035 4～0.424 3 μg范圍內(nèi)線性關(guān)系良好（r＝0.999 5），平均回收率為100.6％，RSD＝1.9％ 。結(jié)論：該法簡便、快速、準(zhǔn)確，適用于參茸健脾膠囊中10-羥基-2-癸烯酸的定量測定，有利于控制參茸健脾膠囊的質(zhì)量。

Objective: To establish an HPLC method for simulataneous determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula. Methods: The HPLC was conducted on discovery Agilent Extend C₁₈ column (250 mm × 4.6 mm, 5 μm). with acetonitrile-0.1% phosphoric acid solution (23:77) as the mobile phase. The detection wavelength was 207 nm, and flow rate was 1 mL/min. Results: There were a good linear relationship of 10-hydroxy-2-decenoic acid within 0.035 4—0.424 3 μg (r = 0.999 5). The average recovery rate was 100.6%, RSD was 1.9%. Conclusion: The method is simple, rapid and accurate for the determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula, and it is expected? for the quality control of Shenrong Jianpi Capsula.