目的 探究甘草次酸及其衍生物TY501對(duì)小鼠巨噬細(xì)胞RAW264.7增殖的影響。方法 以小鼠巨噬細(xì)胞RAW264.7為靶細(xì)胞，設(shè)置空白組，脂多糖（LPS）1 μg/mL處理組（模型組），LPS 1 μg/mL加80、40、20、10 μg/mL藥物處理組（受試藥物TY501，對(duì)照藥物潑尼松龍、甘草次酸、吡羅昔康），每組設(shè)置4個(gè)復(fù)孔在給藥刺激24 h后按照CCK-8試劑盒說明測定各藥物對(duì)細(xì)胞增殖抑制率。結(jié)果 TY501半數(shù)抑制濃度（IC₅₀）為233 μg/mL，潑尼松龍IC₅₀為1 571 μg/mL，甘草次酸IC₅₀為31 μg/mL，吡羅昔康IC₅₀為14 187 μg/mL。結(jié)論 甘草次酸對(duì)RAW264.7巨噬細(xì)胞的增殖具有明顯的抑制作用，甘草次酸衍生物TY501也可抑制小鼠巨噬細(xì)胞RAW264.7的增殖，在同等質(zhì)量濃度條件下其對(duì)小鼠巨噬細(xì)胞增殖的抑制程度強(qiáng)于潑尼松龍，弱于甘草次酸，表明甘草次酸及其衍生物TY501對(duì)于小鼠巨噬細(xì)胞RAW264.7增殖的抑制作用可能是其發(fā)揮抗炎作用的機(jī)制之一。

Objective To investigate the effect of glycyrrhetinic acid and its derivative TY501 on the proliferation of murine macrophage RAW264.7. Methods Murine macrophage cells RAW264.7 were used as target cells, and the negative control group with only RAW264.7, model group with LPS 1 μg/mL treatment, and treatment groups with LPS 1 μg/mL plus 80, 40, 20, and 10 μg/mL of each drug (test drugs TY501, control drug prednisolone, glycyrrhetinic acid, and piroxicam) were set. Each group set four holes and after 24 h of administration, CCK-8 Kit was used for the determination of cell proliferation rates stimulated by different drugs. Results According to CCK-8 Kit determination results, half of the initial inhibition rate (IC₅₀) of the glycyrrhetinic acid was 31 μg/mL, TY501 IC₅₀ 233 μg/mL, Prednisolone 1 571 μg/mL, and Piroxicam 14 187 μg/mL. Conclusion The glycyrrhetinic acid has significantly inhibition on the proliferation of RAW264.7 as well as TY501, in a certain range of concentration, could inhibit the proliferation of murine macrophages RAW264.7. In the same degree of the concentration the inhibition of TY501 on cell proliferation is greater than those of Prednisolone and Piroxicam while less than that of glycyrrhetinic acid, which indicates the inhibition of glycyrrhetinic acid and its derivative TY501 on the proliferation of murine macrophage RAW264.7 may be one of the possible mechanisms of anti-inflammation.