目的 建立丹參藥材HPLC方法同時(shí)用于指紋圖譜和多指標(biāo)測定。方法 采用Diamonsil C₁₈柱（250 mm×4.6 mm，5 μm），在280 nm波長下，以乙腈-0.05%磷酸水溶液梯度洗脫，測定了19批丹參藥材的指紋圖譜和丹參素、迷迭香酸、丹酚酸B及丹參酮Ⅱ_A 4個(gè)指標(biāo)成分，對指紋圖譜共有峰和相似度進(jìn)行分析，并進(jìn)行聚類分析和主成分分析。結(jié)果 在選定的色譜條件下，得到19批丹參藥材的指紋圖譜，標(biāo)定了12個(gè)共有峰，并建立了丹參藥材的指紋圖譜共有模式，相似度在0.808～0.997。聚類分析結(jié)果將19批丹參藥材分為兩大類，與主成分分析結(jié)果及藥材測定結(jié)果相一致。結(jié)論 不同產(chǎn)地丹參藥材色譜峰的數(shù)目差別不大，但各指標(biāo)成分量差別較大。該方法準(zhǔn)確、可靠，為有效地評價(jià)丹參藥材的綜合質(zhì)量提供了參考依據(jù)。

Objective To establish an HPLC method for Salvia miltiorrhiza radix et rhizoma fingerprint and simultaneous determination. Methods The HPLC method was used with Diamonsil-C₁₈ column (250 mm × 4.6mm, 5μm), under the wavelength of 280 nm, a mixed liquid of acetonitrile-0.05% H₃PO₄ as mobile phase in a gradient elution. The common HPLC fingerprint for 19 batches of Salvia miltiorrhiza radix et rhizoma was established, the contents of propanoid acid, rosmarinic acid, salvianolic acid B, and tanshinone Ⅱ_A were determined, and the common peak and the similarity were analysed with parameter of fingerprint analyses. Results Under the selected spectrum condition, it acquired the fingerprint for 19 batches of Salvia miltiorrhiza radix et rhizoma and 12 common peaks. The 19 batches of samples were classified into two groups based on the results of hierachical clustering analysis. It shows no difference with principle components analysis and the results of simultaneous determination. Conclusion The amounts of the peaks from different habitats were the same, but the contents of components showed significant difference in Salvia miltiorrhiza radix et rhizoma from different habitats. The method is accurate and reliable, and it could provide comprehensive reference for the quality evaluation of Salvia miltiorrhiza radix et rhizoma.

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