目的 研究番瀉葉不同粉體的化學(xué)成分與瀉下作用。方法 以燥結(jié)缺水便秘小鼠為模型，運(yùn)用色素流動(dòng)法，以黑便為指示，通過觀測(cè)小鼠首次排黑便的時(shí)間、稀便點(diǎn)數(shù)等指標(biāo)來比較說明番瀉葉不同粉體的瀉下作用強(qiáng)弱。利用HPLC法測(cè)定番瀉葉不同粉體致瀉主要活性成分番瀉苷A、番瀉苷B，比較各粉體中番瀉苷A、B的量高低，反映藥理活性強(qiáng)弱。結(jié)果 通過小鼠動(dòng)物實(shí)驗(yàn)觀測(cè)到，番瀉葉超微飲片組首次排黑便的時(shí)間明顯提前，比飲片組提前了76 min，比超微粉組提前了18 min；稀便點(diǎn)數(shù)增多，比藥材飲片組多3.7個(gè)，比超微粉組多2.84個(gè)。HPLC法測(cè)定番瀉苷A、B的量顯示，超微飲片中番瀉苷A和B的質(zhì)量分?jǐn)?shù)為0.43%，是藥材飲片中的1.6倍。結(jié)論 番瀉葉3種粉體的水煎液都能明顯增加小鼠腹瀉的發(fā)生率，尤其以番瀉葉超微飲片的通便瀉下作用最顯著，番瀉苷A、B的量最高。

Objective To study the chemical constituents of different powder in Folium Sennae and their cathartic effect. Methods The model of constipation on mice was established by water-deprivation but food-intaking, while pigment flow method was applied and black defecation was taken as the index. By observing the first time of black defecation and the loose stools points of mice, etc, we can explain the strength of making diarrhea by different powder in Folium Sennae. By HPLC to determine sennoside A and sennoside B which were the main ingredients to make diarrhea for comparing the content and reflect the intensity of pharmacological activity of different powder in Sennae Folium. Results For the constipation mice, Sennae Folium ultramicro pieces could shorten the first defecation time obviously, 76 min earlier than Chinese herbal pieces, 18 min earlier than superfine powder. It could also increase the points of loose stools, 3.7 more than Chinese herbal pieces, 2.84 more than superfine powder. Determination of sennoside A and sennoside B by HPLC showed that the content of Sennae Folium ultramicro pieces is 0.43%, 1.6 times as high as Chinese herbal pieces. Conclusion Three kinds of decoction of Sennae Folium powder could significantly increase the incidence of diarrhea in mice, and especially the cathartic effect of ultramicro-fine pieces, in which the content of sennoside A and B is the highest, is much more obvious than that of Chinese herbal pieces and ultro-fine powder of Sennae Folium.

國家財(cái)政部社會(huì)公益類行業(yè)科研專項(xiàng)：中醫(yī)藥行業(yè)科研專項(xiàng)（200807054）