干血點(diǎn)采樣（dried blood spot，DBS），即將全血樣品收集在卡紙上，在血樣采集方法上比傳統(tǒng)方法有一定的優(yōu)勢(shì)。它需求較少的血量，可減少動(dòng)物使用，方便血樣采集、存儲(chǔ)運(yùn)輸，簡(jiǎn)化樣品前處理。到目前為止，DBS與LC-MS/MS技術(shù)聯(lián)用在小分子定量分析中已成為一個(gè)重要的方法，將在藥動(dòng)學(xué)研究中得到越來(lái)越多的應(yīng)用。概述DBS樣品收集，儲(chǔ)存、前處理以及分析方法。

The collection of whole blood samples on paperboard, known as dried blood spot (DBS), offers a number of advantages over conventional blood sampling. DBS requires a smaller blood volume and reduces the number of laboratory animals. It simplified sample collection, storage, shipment, and processing. To date, DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecule drugs. It will get more and more applications in pharmacokinetic studies. This paper summarizes the collection, storage, pretreatment, and analysis for DBS samples.

國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃項(xiàng)目（2010CB735602）