目的 研究白藜蘆醇對人胃癌SGC-7901細胞的影響。方法 SGC-7901細胞體外培養(yǎng)48 h，分為白藜蘆醇低、中、高劑量組（44、88、176 μmol/L），陽性對照組（5-FU 153.8 μmol/L）；陰性對照組（不含藥物同體積培養(yǎng)液），熒光顯微鏡觀察不同濃度的白藜蘆醇對SGC-7901細胞的形態(tài)學(xué)影響；流式細胞儀檢測白藜蘆醇對腫瘤細胞中線粒體膜電位、活性氧的的影響；激光共聚焦顯微鏡觀察白藜蘆醇對腫瘤細胞中鈣離子濃度的影響。結(jié)果 顯微鏡下可見腫瘤細胞染色程度加深，染色質(zhì)聚集、斷裂，產(chǎn)生大小不等的凋亡小體，且隨著白藜蘆醇濃度加大，現(xiàn)象越來越明顯，表明細胞凋亡的比例不斷增加；白藜蘆醇能夠明顯降低腫瘤細胞中線粒體膜電位，隨著白藜蘆醇濃度不斷增加，腫瘤細胞中活性氧也不斷增加，說明白藜蘆醇能夠提高腫瘤細胞中的活性氧水平來誘導(dǎo)其凋亡；白藜蘆醇對腫瘤細胞中鈣離子的濃度有一定的作用，其中高劑量能夠顯著提高腫瘤細胞中鈣離子的濃度，且呈現(xiàn)一定的劑量依賴關(guān)系。結(jié)論 白藜蘆醇通過影響SGC-7901腫瘤細胞線粒體膜電位、活性氧及鈣離子濃度導(dǎo)致腫瘤細胞凋亡，且與劑量相關(guān)。

Objective To investigate the effect of resveratrol on human gastric cancer SGC-7901 cell line. Methods SGC-7901 cell line was cultured in vitro for 48 h. The end concentration of resveratrol low-, mid-, and high-dose groups was 44, 88, and 176 μmol/L, respectively; The end concentration of positive control group (5-FU) was 153.8 μmol/L; Negative control group was administrated with the same volume of culture medium without drugs. Morphology of tumor cells treated with different concentration of resveratrol was identified by fluorescence microscope. Flow cytometry was used to detect the influence of resveratrol on the changes of mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) in tumor cells. After the tumor cell was exposed to resveratrol, the intracellular calcium concentration ([Ca²⁺]_i) was measured with laser scanning confocal microscope. Results According to the morphology, the tumor cell staining was deepened; Chromatin aggregation and fragmentation were observed, and the apoptosis bodies were generated. With increasing the concentration of resveratrol, the phenomenon was more obvious, which indicated that the proportion of apoptosis increased. Resveratrol could reduce MMP of tumor cells, and ROS of tumor cells has also increased with increasing concentration of resveratrol. It indicated that resveratrol could induce tumor cell apoptosis by affecting the content of ROS. The high doses could improve [Ca²⁺]_i significantly in a dose-dependent manner. Conclusion Resveratrol could induce tumor cell apoptosis by affecting MMP, ROS, and [Ca²⁺]_i.

黑龍江省自然科學(xué)基金資助項目（D201043）