目的 考察常用的大孔吸附樹(shù)脂對(duì)金銀花多酚類(lèi)物質(zhì)的純化效果，篩選純化工藝條件。方法 以總酚的質(zhì)量分?jǐn)?shù)和回收率作為指標(biāo)，采用靜態(tài)吸附及動(dòng)態(tài)吸附考察樹(shù)脂的吸附、解吸性能。結(jié)果 NKA-2型樹(shù)脂對(duì)多酚類(lèi)物質(zhì)的吸附能力明顯強(qiáng)于其他樹(shù)脂，其載藥量為7倍樹(shù)脂體積，水洗5倍樹(shù)脂體積，60%乙醇洗6倍樹(shù)脂體積。此條件下，浸膏量減少40%左右，總酚質(zhì)量分?jǐn)?shù)提升近一倍，達(dá)到60%左右，樣品回收率達(dá)到55%。結(jié)論 NKA-2型樹(shù)脂對(duì)金銀花多酚類(lèi)的純化效果較好，適用于金銀花多酚類(lèi)物質(zhì)的分離純化。

Objective To study the process parameters for purification of polyphenols from Lonicerae Flos by commonly used macroporous resins, and to optimize the purification technological conditions. Methods The purity and recovery of polyphenols were taken as indexes, and the adsorption and desorption performance by difference resins were studied by using static adsorption and dynamic adsorption. Results It has been found that NKA-2 resin’s adsorption capacity of polyphenols was the best among those resins. The optimum condition is seven volume times of sample, five volume times of water washing, and six volume times of 60% ethanol washing. Under those conditions, 40% extract was reduced with the purity of polyphenols around 60%, and the recovery rate was 55%. Conclusion NKA-2 resin has a good performance and is suitable for the production of polyphenols from Lonicerae Flos.

國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展規(guī)劃（2009CB523003）；國(guó)家自然科學(xué)基金資助項(xiàng)目（81073033）